动物造模,慢性胃溃疡动物模型 z-bio 2021-11-26 374

　　(1) Breeding method For adult rats, open the incision aseptically, heat it to about 45°C with a 3mm thick 15W electric soldering iron, and cauterize the surface of the glandular serosa for about 5 seconds. Alternatively, the stomach wall is incised, the mucosal surface is directly burned, and then the stomach wall is sutured. After completing the above steps, restore the stomach to its original position and suture the abdominal wall. After the animals wake up, they are raised according to traditional methods.

　　(2) The characteristic of the model is that the surface of the gastric gland serous membrane is burned for 5 seconds, there are obvious white spots on the injured part, there is edema on the mucosa, and obvious bleeding after 6 hours and 12 hours. Two days later, the surrounding tissues (omentum, liver surface, etc.) adhered and basically disappeared, but four days after the operation, the mucosal surface of the burned area reappeared edema and mild bleeding, forming ulcers. A small white coating is seen on the bottom. Direct burning of the mucosal surface is accompanied by local spotting, bleeding, ulcers, necrosis, and damage to certain tissues. After 4-6 hours, the most serious is at 12 hours and 7 days after surgery, the mucosal epithelium regenerates and the mucosa. appear. The surface of the ulcer can heal in 2-4 weeks.

　　(3) In comparative medicine, a rat gastric ulcer model was created by directly burning the surface of the glandular stomach. The induced ulcer is large and deep, and its main morphological characteristics are basically similar to those of human chronic gastric ulcers. The modeling success rate is high, and the incidence of ulcers reaches 100%. After direct cauterization, 75 mg/kg body weight of cortisone is administered to the animal every day. Continuous administration can prolong the pathological process and cause gastric ulcers to last for 200 days.