应激性胃溃疡动物模型,动物造模 z-bio 2023-01-31 527

　　(1) Feeding method Anesthetized adult experimental rats without ascites intraperitoneal injection of 30 mg/kg body weight of pentobarbital sodium after fasting for 24 hours are placed in a hot water bath at 80-92°C for 10-18 seconds. The burning area is controlled at 30%-50%. After 3 to 48 hours, blood was collected and necrosis occurred immediately. The method of measuring and evaluating the sample site and the gastric mucosal injury index is the same as that of the animal model that inhibits water immersion to induce stress gastric ulcer, and the content of gastric mucosal cortisol (GC) is measured.

　　(2) Model characteristics: The model animal injury index increases at 6 hours after burns, reaches a peak at 24 hours, and reaches a peak at 48 hours. The gastric mucosal cortisol (GC) content also increased significantly at 6 hours after burns, and peaked at 12 and 24 hours. It is at the same peak as 48 hours. The gastric mucosal injury index is positively correlated with the gastric mucosal cortisol (GC) content.

　　(3) Comparative medical burns are also a source of stress. When an animal is burned by hot water in a large area, it will increase the excitement of the sympathetic nervous system and adrenal medulla, cause gastric vasoconstriction, reduce the mucosal barrier function, and eventually cause various stress factors, and may also induce stress ulcers. The replication method of this model is rarely used recently because it is difficult to control, the influencing factors are more complicated, and the induced lesions are different from human peptic ulcers.