动物造模,十二指肠溃疡动物模型 z-bio 2021-03-23 803

　　(1) Feeding method Adult rats were fasted with water for 16 hours, anesthetized by intraperitoneal injection of sodium pentobarbital, fixed, and then pressed into the coat with electric clippers. The area was slightly larger than the burned area. Pat the bald area evenly with a gauze ball soaked in 20% sodium sulfide. After the hair has melted, rinse the depilatory with warm water, absorb the moisture with gauze, and then blow dry with a damp cloth to cover the depilatory skin. Burn the surrounding skin. Spread 3% napalm evenly on the burned skin surface at a weight ratio of 0.01 ml/kg, and ignite for 30 seconds to burn in a stable airflow environment. Among them, the method of constructing 3% napalm is to accurately weigh 3 g of napalm powder, add gasoline to 100 ml, put it in a mashed tank and stir thoroughly until the oil powder is evenly mixed in the gel . Immediately after the injury, anesthetize each part to fully expose the stomach and duodenum, separate the greater omentum and part of the mesentery, and cut the duodenum along the larger curvature of the stomach, using the open duodenum . The contents were washed with physiological saline at 4°C, and the damage of the duodenal mucosa was observed with the naked eye. At the same time, some tissues were collected for optical and electron microscopic examination of histomorphology.

　　(2) Model characteristics After burns, the duodenal mucosa of the model animals developed ulcers, local erosions and necrosis. Electron microscopic examination showed that the entire duodenal villi were swollen and thick, with fuzzy surface folds, rough grooves, misalignment, and some upper tissues of the villi ruptured. Some surfaces show spots or band-like defects. Two hours and five hours after the injury, intestinal villi edema continued to exist, but the degree of injury expanded and worsened. Eight hours after the injury, villous edema still exists, most of the villi have necrotizing lesions of varying degrees and areas, and some villi are divided into two parts. Twenty-four hours after the injury, the damage to the villi structure was very serious and reached its peak. Some villi are divided into several fragments, fragments, some necrotic villi fused to form necrotic lesions or honeycomb or hollow, and accumulated okara and other substances. 72 hours after the injury, the edema has basically disappeared, but the surface structure is still chaotic, and villous necrosis still exists.

　　(3) When comparing burns, a variety of factors can cause a strong stress response to the body. Increased excitement of the sympathetic nervous system and adrenal medulla will cause strong constriction of mucosal blood vessels, decreased local blood flow and decreased mucosal barrier function, resulting in stress duodenal ulcer. This model not only causes damage to the intestinal villi, but also induces ulcers in the gastric mucosa, which is determined by common pathophysiological basis. Generally speaking, the total incidence, duration and severity of duodenal mucosal lesions caused by burns are more sensitive and severe than gastric mucosal lesions. This model is suitable as an animal model for studying gastrointestinal mucosal damage caused by severe burns and how to prevent and treat them.