动物造模,腹腔感染模型 z-bio 2022-11-14 946

　　(1) Breeding method Adult male rats weighing 250 to 270 g were used, and the abdominal infection group was subjected to intestinal fistula surgery. Before the operation, the rats were fasted for 8 hours, and anesthetized by intraperitoneal injection of 100 mg/kg body weight and fixed in the supine position. Operating table: curettage the surgical site of the abdomen, disinfect the iodine regularly, make a midline incision in the middle and lower abdomen, cut 1/3 of the circumference of the small intestine from the tail of the ileum 20 cm, and cut the right abdominal wall of the medial corneal limbus , Intestinal wall. There are 3-0 sutures 1 cm above and below the perforation. Divide a silk thread into two layers to close the abdomen. 48 hours after modeling, the model animals were sacrificed and the peripheral blood white blood cell count was measured. At the same time, peripheral blood and ascites were cultured in a blood culture dish for 48 hours to identify the number of colonies and bacteria. Species. Model animals are 2 cm above and below the fistula. A part of the intestine with fistula was removed, and routine tissue sectioning, HE staining and optical microscopy were performed.

　　(2) Model characteristics 12 hours after the operation, the WBC of the peripheral blood of the model animals began to rise, and the ascites culture was mainly E. coli and group D streptococcus. After 16 hours, the model animal may die. The animal’s intestinal tract was significantly dilated, with congestion and edema, and the intestinal wall fragile and bleeding. Under an optical microscope, the serosal layer, muscular layer and lamina propria propria of the small intestine were all hyperemia, accompanied by inflammatory cell infiltration, mesenteric vasodilation and hyperemia, and peripheral blood inflammatory cell infiltration. Blood vessels. Compared with models replicated by other methods, the peritoneal infection model replicated by this method has lower mortality, relatively better model stability, and is again suitable for bowel surgery.

　　(3) Comparative medicine. This model can simulate the symptoms of abdominal infections in clinical patients and suffer from typical peritonitis. The manufacturing method is simple, the result is stable and reliable, and it is convenient for reoperation. Although the model is used for experimental research and observation, it can still achieve the purpose of simulating clinical intestinal fistula and has great practical value.