动物模型 z-bo 2020-08-04 552

　　[Modeling mechanism] The surface temperature of the tree shrew's foot pad is lower than 32℃, which is suitable for the growth of leprosy bacteria. After inoculation of 50×10000/foot pad, after an average infection period of 550 days, the leprosy bacteria can multiply to 25×1000000000/g tissue.

　　【Features of the model】Bacillus leprosy was inoculated through different ways of foot pads, ears, nose, and testes. Leprosy granuloma damage can be seen in histopathology at the inoculation site. The granuloma contains colonies, macrophages and a few foam cells, similar to the boundary of human leprosy. Tumor-like lesions. The bacterial index is as high as +++++～++++++, and the morphology index is as high as 40%. The foot pad is the most suitable site for inoculation. Intravenous inoculation of Leprosy Bacillus can spread to internal organs, but it did not cause obvious pathological changes in leprosy.

　　[Model Evaluation and Application] Tree shrews are lower primates, closely related to humans, with a high reproduction rate and rapid generation changes. Tree shrews are easy to domesticate and manage and are easy to operate in experiments. They have been captured from the outskirts of Kunming in China. Tree shrews may provide a new option for the development of leprosy infection models.