多器官功能衰竭综合征,动物造模 z-bio 2021-12-30 736

　　(1) Method of replication Using 6-8 weeks old KM mice, each mouse was inoculated with Vibrio vulnificus (VV) through different routes (intraperitoneal, intramuscular and subcutaneous injection) 0.2 ml (4.74 X 1000000). After the animal died, the blood, heart, lung, liver and muscle tissues of the experimental mice were dissected, inoculated into liver digest agar (pH = 7.6), incubated overnight, and the corresponding colonies were observed. confirm. Ultra-thin section electron microscope observation: Collect the heart, lung, liver and muscle tissue (0.5 cm x 0.5 cm x 0.5 cm) of model mice, and place them in glutaraldehyde fixative to prepare ultra-thin section, transmission electron The microscope is used. And observation. The mouse model shows that the pathological features of multiple organ dysfunction syndrome have been confirmed. Therefore, a mouse model of multiple organ failure syndrome has been established.

　　(2) Approximately 58.3% of the characteristics of model animals died within 4-6 hours of Vibrio vulnificus (VV) and mouse mortality model mice reached 100% within 8 hours after inoculation. May be reached. .. The blood, heart, lung, liver of dead mice, which is the muscle tissue cultured overnight, that is, the growth of Vibrio? The results of the Vibrio discovery have been confirmed. Ultrastructural electron microscopy showed perivascular exudation, interstitial edema, mitochondrial swelling, and reduction or loss of cr in the liver and muscle tissue. In these tissues and organs, Vibrio vulnificus infects mice’s ultrastructural changes, the body causes multiple organ dysfunction syndrome (MODS) in mice, and ultimately the histopathological characteristics of multiple organ dysfunction syndrome systemic damage Repeated mouse model.

　　(3) Comparative medicine Balnificus Vibrio has been confirmed by several research reports to cause intestinal damage in the body. Humans can be infected with Vibrio balnificus by eating raw oysters or contacting wounds with contaminated seawater. The main clinical symptoms of the patient are diarrhea and wound infection. Suffering from liver disease, alcoholic cirrhosis, and AIDS have a history of primary sepsis and high mortality. Clinically, patients usually initially develop blood blisters and cellulitis on the skin of the lower extremities, rapidly develop sepsis, and may die of irreversible septic shock. Most patients will die within 1→2 days after the onset of illness. The rapid death and high mortality of Vibrio vulnificus have been proposed, and higher requirements have been put forward for the establishment of corresponding animal models. Through experiments, smooth colonies and Sayamakukabu are signs of pathogenic bacteria, and it has been seen to be very pathogenic in mice. In this model, through electron microscopy, it was confirmed that the mouse blood, heart, and lungs, namely Vibrio vulnificus, were present in muscle cells. This indicates that Vibrio vulnificus can be injected from the abdomen to the heart, lungs, liver, and blood throughout the body. The regeneration of the abdominal cavity, skin and muscles is one of the most direct evidence that Vibrio varnificus causes sepsis. Using this model, we can observe the existence of Vibrio varnificus, and also confirm that Vibrio varnificus may cause changes in the ultra-fine structure of some organs and tissues. Significant common features are mitochondrial swelling and reduction or destruction of collagen fibers in tissues and cells, such as myocardium, lung, liver and muscle, hyperinterstitial edema, and severe capillary exudative cristae loss. The cells showed coagulation-like necrosis. The main necrosis caused by Vibrio vulnificus, obviously, causes damage to the heart, liver, lungs, and other vital organs throughout the body. The practical value of the Vibrio balnificus multiple organ failure model established in this way.