动物造模,豚鼠支气管哮喘动物模型 z-bio 2021-03-25 1135

　　(1) Model: After 5 to 7 days of adaptive reproduction, adult guinea pigs are injected intraperitoneally with 10% ovalbumin saline at a weight of 200 mg/kg and inhaled intraperitoneally on the 15th day. Spraying 5% egg white albumin saline to induce asthma attacks; the control received regular intraperitoneal injections and sprayed saline instead of inhalation of the antigen solution.

　　(2) Model features Guinea pigs show shortness of breath immediately after inhaling egg protein. In severe cases, the breathing rhythm becomes slow or irregular, accompanied by mild purple osis, quadriplegia, slow movements, unresponsiveness, and loss of luster. Thirty days after modeling, the total number of eosinophils in guinea pig model blood, bronchoalveolar lavage fluid (BALF) and BALF cells was significantly higher than before modeling. When measuring the responsiveness of the airway to histamine, as the inhaled histamine concentration increases, the animal model of airway pressure will gradually increase. Microhistopathological examination showed that the bronchi and alveolar cavity of the model animals showed a significant increase in exudation; and the eosinophils of the inflammatory cells in the bronchial wall and peribronchial tissue, especially the bronchial mucosal edema, infiltrated the spheroids.

　　(3) Comparative medicine Bronchial asthma is one of the most common diseases in the respiratory system. Its etiology, prevention and treatment are becoming more and more understood in the past ten years, but its mortality rate is still high. Therefore, research on this is very active. The target organ for allergy to guinea pigs is the lungs, which may cause immediate and delayed bipolar asthma, making it a commonly used asthma model. There are about bronchial asthma, and the theory of airway inflammation is widely accepted. There are many theories and asthma is a chronic airway inflammatory disease involving a variety of inflammatory cells and inflammatory mediators. In order to reflect the basic characteristics of bronchial asthma, this model uses airway responsiveness and eosinophilia as the observation indicators for modeling technology research. The resulting guinea pig model is an asthma model with unique symptoms of asthma, changes in ventilatory function, and pathological changes that meet recognized standards.