动物造模,静脉注射氯化汞肾病模型 z-bio 2021-03-26 361

　　(1) Reproduction method For adult male mice weighing 25 to 30 g, HgCl2 is prepared into 0.125 g/L Hg2 + solution and physiological saline, and then HgCl2 ml/kg body weight is injected from the tail vein of the mouse at a dose of 10 It is 1/4 of LD50). After anesthesia for a predetermined time, blood was drawn from the femoral artery to measure serum creatinine and urea nitrogen, and the kidney was quickly taken out at the expense of the animal, washed with saline and sucked dry. The content of lipid peroxide (LPO) and trace elements (such as mercury, calcium, iron, zinc, and copper) in the treated kidney; collect some kidney tissue specimens, fix them in a fixative, and then soak them in saline In water to form normal tissue sections, HE stained, and observed under an optical microscope.

　　(2) Model characteristics After the injection of mercury chloride, the serum creatinine and urea nitrogen of the model mice increased significantly, and reached the peak value on the first day after the injection. With the passage of time, serum creatinine and urea nitrogen gradually decreased, but the time on day 7 was still significantly higher than that of normal mice. After injection of mercury chloride, the level of LPO in the kidneys of model mice increased significantly. At various subsequent time points, the concentrations of mercury, calcium, iron, zinc, copper and other metals in the mouse kidneys were significantly higher than those in the control group. Injection of mercuric chloride. Although there is a relationship between time and effect, starting from the 5th day after injection, the concentration may return to normal. Observing histopathological blood under a microscope, it was found that one day after injection, the renal tubular epithelial cells in the kidney tissue of the model animals were severely degenerated, some cells were necrotic, and some renal tubules were obviously degenerated and enlarged, and a large amount of protein was found in the lumen.

　　(3) In clinical comparative medicine, misunderstanding of mercuric chloride can cause acute corrosive gastroenteritis and necrotizing nephropathy. It can also lead to anuria, retention of nitrogen-containing metabolic end products, and acute renal failure in patients. This model uses direct intravenous injection of mercuric chloride into the blood circulation of model animals. This leads to a rapid increase in serum creatinine and urea nitrogen, which leads to degeneration, necrosis and protein casting of renal tubular epithelial cells. Model animals; At the same time, the level of renal lipid peroxidation LPO (LPO) also increased significantly, indicating that there is a significant dose-effect relationship. The model confirmed that mercuric chloride has obvious nephrotoxicity. It promotes lipid peroxidation in the body and may cause the metabolism of calcium, iron, zinc, copper and other trace elements. These symptoms may be one of the key factors for mercury chloride. Cause kidney damage.