动物造模,膀胱炎动物模型 z-bio 2021-03-26 842

　　(1) Breeding method Inject cyclophosphamide solution into a New Zealand female rabbit weighing about 1.5 kg at a dose of 8.0×10-2 g/kg from a guinea pig vein once. After 6 hours, use 0.1, (Xinjieerjie) for rabbits The skin around the vulva is disinfected, and it is injected into the bladder catheter under aseptic conditions, urine is sucked with a syringe, and then 1 ml of Escherichia coli containing 1,000,000 E. coli/ml is injected, and the culture solution is extracted and injected. Insert it into the bladder through the catheter and pull it out of the cannula. Animals can drink and eat freely. After 24 hours, observe the frequency of urination of the animals and record the number of urinations within 12 hours. At the same time, blood was drawn for blood smear, and the white blood cells were observed and counted under a microscope. The experimental rabbits were sacrificed by injecting air into the ear vein, the abdominal cavity and pelvis were opened surgically, the bladder was exposed, the urine was sucked under aseptic conditions, the urine drops were removed and placed upside down, and the urine deposition was observed under a microscope. Cut the bladder and cut the ureter between the two ureters. Fix the full-thickness tissue of the bladder wall with 10% formaldehyde, and perform normal tissue sectioning, HE staining and microscopic observation.

　　(2) Model features Cyclophosphamide plays a major role in the formation of cystitis in this model. Cyclophosphamide can retrogradely transport nerve growth factors in the body on nerve axons, leading to changes in bladder function and inflammation. Escherichia coli infection plays a very important role in the formation of cystitis in model animals. Abnormal papG alleles of Escherichia coli can induce the first stage of acute cystitis in rabbits. 24 hours after the injection of E. coli culture medium, the urination frequency of the model animals was significantly increased, the differentiation of leukocytes was well observed under the blood smear microscope, and no significant reduction or proliferation of leukocytes was observed. When observed with the naked eye and under a microscope, the urine of the model animals was yellow and obviously turbid. Under the microscope, there are 70-80 red blood cells/HP, 2-3 neutrophils/HP and 2-3 epithelial cells/. There is HP in the urine. , And you can see many cell lysates. Microscopic pathological observations showed that the bladder mucosa was obviously damaged, sustained damage, shedding from the submucosa, and a lot of bleeding and congestion were seen in the bladder tissue. The modeling method is simple, convenient to operate, low in cost, and stable in pathological performance of the model.

　　(3) Comparative medicine Acute cystitis is a clinically common human lower urinary tract infection. After illness, the clinical symptoms are complex and diverse. In addition to frequent urination, urgency and pain, the bladder also has a burning sensation and drainage. .. After infusion, lower abdomen or lower back pain, persistent pain or tight urethra, common diseases, etc. Failure to timely and effective treatment may aggravate the patient's retrograde infection and other complications. Currently, there are more methods and better results available for the clinical treatment of acute cystitis. However, with the recent emergence of bacterial resistance, the prevalence of the disease tends to increase. Therefore, the establishment of an ideal animal model of acute cystitis has excellent comparative medical significance. This model introduces cyclophosphamide and E. coli into model rabbits through ear vein injection and bladder infusion, respectively introducing frequent urination, abnormal urine composition, bladder mucosal damage, extensive interstitial hemorrhage and congestion, and other pathological symptoms. An animal model was established. This model can be used to study drug treatment and drug screening for retrograde bladder infections.