动物造模,膀胱结石动物模型 z-bio 2021-10-27 367

　　(1) Breeding method Breed 55-60g pups in a cage. 5% terephthalic acid (TPA) was added to the standard rat diet, and the experimental diet was used to feed the rats for 14 consecutive days. Give the rats free water. On the 14th day of modeling, the animals were anesthetized with sodium pentobarbital, the bladder was surgically removed, and placed in a 10% formaldehyde solution for normal tissue sectioning, stained with HE staining and Von Kossa calcium marker, and stained. Optical microscope.

　　(2) Model characteristics After feeding 5% TPA diet for 14 days, there are calcified bodies of various sizes and numbers in the bladder cavity of model rats. The calcified object is surrounded by a layer of uniform thickness. The salt in the black calcium body is in the form of unevenly distributed particles, some of which converge into clusters. The model creation method is simple and convenient, the model creation time is short, the model success rate is high, and the repeatability is good.

　　(3) Comparative medicine Urethral calculi is a common disease of the human urinary system in clinical practice. Due to its complex etiology, animal models similar to clinical human urolithiasis have been established to study the development of urolithiasis. Both mechanisms and preventive measures are very important. In this method, feeding rats with phthalic acid as a stone agent can induce bladder stones in rats in a short time, and the pathological characteristics of the model animals are stable and the model is correct. Similar to human urinary tract stones. This model has practical value in clinical research on the etiology of urinary calculi, drug treatment and drug screening.