子宫胎盘缺血模型,动物造模 z-bio 2022-02-06 467

　　(1) Replication methods At present, various animal model studies mainly use experimental animals, such as pregnant dogs, pregnant rabbits, macaques and baboons. Pregnant rabbits (dogs) and other experimental animals were anesthetized by intravenous injection of sodium pentobarbital at a dose of 30 mg/kg body weight. After anesthesia, the animal was put back on the operating table, the abdominal operation site was disinfected regularly, and the hair was removed along the midline of the abdomen. An incision is made in the lower abdomen, the abdominal aorta is separated after entering the abdominal cavity, and then the abdominal aorta is clamped with arterial clamps. When the uteroplacental perfusion pressure drops, the peripheral blood pressure rises rapidly and remains until the obstruction of the abdominal aorta is completed. In a pregnant rabbit model, it was found that after the abdominal aorta was clamped, the blood pressure would rise the next day, and it could be maintained until abortion. At the same time, pregnant rabbits will have proteinuria, glomerulonephritis and fibrin. Capillary deposits and visible fetal development disorders. A study of infants holding the abdominal aorta found that after a decrease in blood flow in the abdominal aorta in the later stages of pregnancy, abdominal vascular resistance increases and blood increases, and the infant's blood pressure may increase by about 30%. The increase in volume during pregnancy will occur later. Some pregnant chicks may also experience proteinuria after clamping the abdominal aorta, resulting in decreased plasma renin activity, hyperuricemia, fetal growth retardation and high fetal mortality.

　　(2) Model features Obstruction of the animal’s abdominal aorta during pregnancy can induce hypertension in pregnant dogs, pregnant rabbits, macaques and chickens, but these experimental animals have found the same intervention factors in non-pregnant animals. High blood pressure will not occur. Therefore, the effect of this kind of hypertension may be related to uterine placental ischemia caused by the closure of the abdominal aorta of pregnant animals, thereby reducing the perfusion pressure of the uterine placenta. Using the model established in this way, most model animals can show symptoms of preeclampsia, such as proteinuria, hyperuricemia, and fetal development delay. However, it was found in the study that some model animals would block the lower abdominal aorta. It will inevitably lead to the development of high blood pressure.

　　(3) Comparative medicine. This method is based on the establishment of a model. In normal early pregnancy of pregnant women, patients with pregnancy complicated by hypertension, uterine placental ischemia, and hypoxia are clinically and clinically affected by the syncytiotrophoblast cell membrane. erosion. Decidua and spiral arteriosclerosis can lead to the placenta of the uterus and placenta; however, in patients with pregnancy-induced hypertension, the nutrient membrane is insufficient to erode the spiral artery in the first trimester. The spiral placental artery appears as a lumen, and in some cases , There may be stenosis, arteriosclerosis and necrosis. However, the cause of preeclampsia may not only be related to this factor, but also related to the simultaneous involvement of the maternal vascular system. Therefore, it is not clear that the obstruction of the aorta that induces hypertension is completely or partly caused by insufficient perfusion of the uterus and placenta, and a model of pregnancy congestive stress syndrome cannot be replicated in this way. This feature limits this. The actual scope of the model. This model is suitable for studying the pathophysiological mechanism of a series of complications after pre-eclampsia, but the research on the etiology and etiology of pre-eclampsia is limited.