动物造模,角膜新生血管模型 z-bio 2021-03-29 257

　　(1) Breeding method When using copper chloramine hydrochloride (50 mg/kg body weight) and chlorpromazine (10 mg/kg body weight), New Zealand rabbits weighing 2.5 to 3.5 kg and weighing more than 4.5 kg are unlikely to induce corneal neoplasia Blood vessel formation. . Intramuscular injection and local anesthesia with 0.5% propoxybenzoin hydrochloride. Wipe off excess water with a cotton swab. The filter paper with a diameter of 7 mm is immersed in a sodium hydroxide solution with a concentration of 1 mol/L for 10-20 seconds, and then it is placed in the center of the rabbit cornea for 2 minutes. Add 25μl of 4 mol/L sodium hydroxide solution every minute, point to the center of the filter paper, and let it stand for 1 minute. Then rinse with 15 ml of balanced salt solution for 60 seconds.

　　(2) Model characteristics: Within 2 hours after rabbit eye injury, the vascular network of the corneal valve annulus will expand and increase permeability. Inflammatory cells in the blood vessels accumulate, attach and exude, and exhibit chemotaxis in the burning area. After 27 hours, the vascular network of the ring grew vascular sprouts and extended toward the burned area. At the same time, it continues to match the branch. It grows into the cornea within 72 hours and reaches the burning area within 7 days. One week after chemical burns, corneal neovascularization begins to differentiate and rebuild, and many small blood vessels are absorbed shortly after formation. Within one month, there were few large blood vessels, edema and inflammatory cells disappeared, and the cornea was basically transparent, with only turbidity caused by blood vessels.

　　(3) Comparative medicine The principle of this model method is to use alkaline chemicals to cause alkaline burns of the cornea, thereby inducing corneal neovascularization, which is a model of inflammation. The cornea infiltrates the infiltrating polymorphic neutrophils into the corneal stroma, after which new blood vessels proliferate. This model reproduces the pathological condition of corneal alkaline burn very realistically, and is an important method for studying the mechanism and treatment of inflammatory angiogenesis. It is difficult to quantitatively analyze its shortcomings, which makes the cornea more susceptible to bacterial keratitis and corneal ulcers.