动物造模,硒性白内障模型 z-bio 2021-11-11 425

　　(1) Reproductive method Inject 0.16% sodium selenite solution into the abdominal cavity of 12-day-old baby rats weighing about 25g, weighing 0.26ml/kg, and rat cataracts.

　　(2) Model features The lens of the model animal was completely turbid 5 days after modeling, and the lens of the model animal was still completely turbid 30 days after modeling. Injecting 0.1% sodium selenite solution into the abdominal cavity of model animals for 3-5 days can quickly cause severe nuclear cataracts. The model replicated in this way is faster than the animal model of glucose cataract (1-2 months). The model copy method is simple, low cost, and reproducible.

　　(3) Compared with medicine, rabbit crystals and liver glutathione peroxidase activities that have been deficient in selenium for a long time have been reduced by 85% and 100%, respectively, but second-generation animals lacking selenium are more likely to develop cataracts. On the contrary, using high levels of selenium can also directly induce cataracts in animals. The method can be used to rapidly induce severe nuclear cataracts in model animals in a short time. This model mainly reflects the acute cataract process of animal lens nucleopathy, and can be used for clinical drug treatment and drug screening of human cataract. Acute animal model.