动物造模,紫外线照射白内障模型 z-bio 2023-02-07 586

　　(1) Reproduction method Healthy adult guinea pigs, the right eye is the irradiated eye, and the left eye is the control eye. Use 1% atropine solution to inflate the pupils of both eyes at least twice a week to dilate the pupils by at least 5 mm. The guinea pig was placed in a metabolic cage, and the irradiated right eye was irradiated to the ultraviolet lamp for 8 hours a day for 8 months.

　　(2) Model characteristics Model animals have different crystal turbidity after 6-8 months of exposure to ultraviolet light. The opaque areas are mainly located in the center of the lens and the anterior and posterior cortex. They are small dots, vacuoles, flakes or feathers. Under an optical microscope, the lens of the eye irradiated by ultraviolet rays usually shows the expansion of the lens fiber, and the difference in the distribution thickness is obvious. There is almost no fibrous layer in the obvious lesion area, and vacuolar degeneration and tearing can be seen. You will see small clusters of eosinophils. Body and vesicle cells. The crystal nucleus is not uniformly dyed, and the crystal epithelium becomes thicker. Electron microscopy showed that the crystalline fibers were obviously swollen, and the inter-fiber gaps were obviously widened.

　　(3) Comparative medicine 70% to 80% of long-wave ultraviolet rays can pass through the cornea, and almost all crystals absorb it. Therefore, it can be observed that people's glasses are exposed to chronic long-wave ultraviolet rays for the rest of their lives, and the incidence of human cataracts increases with decreasing latitude or increasing altitude. In addition, long-term, long-term accumulation of high-intensity ultraviolet rays will not only produce more free radicals, but also reduce the content of crystalline glutathione. The result of these combinations is that the crystal loses its protective mechanism, converts free radicals into fluorescent substances, participates in cataracts and accelerates its development. generation. This model can be used to study the mechanism and prevention methods of cataract formation caused by long-term, long-term high levels of ultraviolet radiation.