动物造模,导角膜新生血管模型 z-bio 2021-12-02 305

　　(1) Breeding method New Zealand rabbits weighing 2.5-3.5 kg, flush the conjunctival sac and wash the eyes with cocaine (12.5 g/L) 3 times before surgery. The eyelid opener is used to open the eyelid. Using a two-corner needle (3/8), thread a 1-0 silk thread on the upper cornea to form a three-needle suture. The distance between the ends of the sutures is approximately 2.5 mm. The length of the suture embedded in the corneal stroma at the end of the cornea is approximately 3.0 mm. The length of the line remaining on the surface of the cornea is about 1.0 mm. The growth of new blood vessels was observed on the 3rd day after the operation, and the growth of new blood vessels was observed on the 18th day.

　　(2) Model features: Corneal neovascularization grows limitedly along both sides of the suture line. Growth is dominant along the suture direction. The main mechanism is the suture reaction, which manifests itself in the form of inflammation.

　　(3) The advantage of this model copying method in comparative medicine is that it is easy to operate. This helps to observe and compare the growth of new blood vessels. Compared with other methods, it also eliminates the influence of chemicals on preventive and therapeutic drugs, reduces corneal damage, and makes the prevention of postoperative infections easier.