动物造模,Ⅲ型变态反应模型 z-bio 2022-02-01 307

　　(1) Replication method Dissolve rabbit anti-bovine serum albumin antiserum in PBS, pH 7.2, and put 100μl into rabbit eyelid conjunctiva four times. On the 9th day after the last injection, 0.1 ml of 0.15 mmol/L fluorescein sodium isothiocyanate bovine serum albumin (F-BSA) was injected intravenously. Thirty minutes later, intravenous injection of bovine Y globulin 50 mg/kg body weight induced allergic conjunctivitis.

　　(2) Model characteristics To form this model, the antigen must be continuously present in the animal. The antigen combines with the corresponding antibody to form a free immune complex. Under certain conditions, the composite is deposited on the basement membrane. It is the membrane of the blood vessel wall or interstitial space and is activated by activation. Complement, neutrophils or platelets cause inflammatory changes at the deposition site.

　　(3) Comparative medicine Rabbits may react because guinea pigs, rabbits and rats will develop type III allergies, and rabbits will form high levels of sediment during the sensitization process, which is the highest. Allergies are related to the level of blood sedimentation in the body. This model has a simple replication method, high success rate and stable Arthur reaction, so it is suitable for screening type III allergy treatment.