动物造模,视神经牵拉伤模型 z-bio 2021-11-09 795

　　(1) Regeneration method. Anesthetized sodium pentobarbital with a body weight of 30 mg/kg was injected intraperitoneally into a guinea pig weighing about 700 g. The extraocular muscle was cut with an operating microscope, and the corneal ring was cut through 360° to release it. . It exposes the conjunctiva, extraocular muscles and optic nerve and separates them from surrounding tissues. Take a sterile sling, cut a 5 mm long crack at the midpoint, place it on the eyeball, and fix it with silk thread so that the optic nerve passes through the crack from the back. Fix the guinea pig on the stereotactic fixed frame, and adjust the head support frame to match the direction of pulling the optic canal axis. Both ends of the sterile sling fixed on the ball are connected to the cylinder of the pulling device with silk threads, and the pulse generator generates a constant voltage to move the piston and transmits it to the pulling device through a pulley system. The transmission path is cylinder 1, suspending the optic nerve so that it can be pulled until it is pulled.

　　(2) The method of model features causing optic nerve traction injury can be divided into traction force that coincides with the axis of the optic canal and traction force that hangs over the axis of the optic canal. The former can accurately cause diffuse axonal injury. Both of the amputation injuries caused by optic canal fractures can more accurately quantify the degree of injury. However, experimental research using this model method may have better clinical significance.

　　(3) Comparative medicine The mechanism of optic nerve injury is very complex, including primary optic nerve and vascular injury, such as shearing, compression and extension, and secondary ischemic injury caused by the swelling of the optic nerve in the optic canal. I will... Most animal models do not have an optic canal and can only simulate one or two injuries, but they cannot completely simulate it. The ideal optic nerve injury animal model, as the evaluation principle of the human disease animal model, must meet the following requirements: (1) The injury device is simple and easy to operate, few surgical procedures, little damage to experimental animals, and low animal mortality. (2) The damage rate is high, and while preventing damage to the central retinal artery and choroidal capillary layer, it may cause accurate optic nerve damage, and the degree of damage can be accurately quantified. (3) Since the injury state is as close as possible to the clinical injury state, experimental research has clinical significance.