动物造模,创伤性关节软骨损伤修复动物模型 z-bio 2021-11-15 330

　　(1) Reproduction method Anesthetize 4-5 months old New Zealand rabbits with intraperitoneal ketamine of 50 mg/kg body weight, and then fix them on the surgical plate. An incision is usually made on the outside of the sterile surgical site. Finish the articular cartilage on the surface of the bone, expose the distal femur on the side of the animal bone, and then use a manual drill to form a full-thickness cartilage defect with a diameter of 4-6 mm on the surface. Articular cartilage. One side of the defect site was filled with test material as the experimental group, and the other side was filled with collagen gel as the control group. After the operation, the experimental rabbits were routinely housed in rabbit cages and moved freely. After the operation, observe whether the animal’s knee joints have contractures, adhesions, and neoplasms, and observe the joint pain, stiffness and dysfunction of the animal model. The model animals were sacrificed 1 to 24 weeks after the operation, and samples were taken for histopathological observation. The specimens were made into normal pathological sections, stained with toluidine blue and HE, and placed under an optical microscope for histological observation. Observe whether the animal’s knee joints have contractures, adhesions, slow and progressive degradation of articular cartilage, synovial edema, hyperemia and color.

　　(2) Model features This model is mainly used in new areas of biomedical science development in recent years. Tissue engineering repairs defects in articular cartilage, which is the science of preparing new tissues to replace defective tissues and regenerate new tissues. Articular cartilage is hyaline cartilage with low tissue metabolic activity. It is difficult to repair or replace it with fibrocartilage or fibrocartilage due to cartilage damage or loss caused by trauma or surgery. It is a tissue for repairing articular cartilage defects and has a great impact on the application. The animal model of traumatic articular cartilage injury established by this method can be used to observe the effect of tissue engineering repair on animal cartilage cells.

　　(3) In comparative medicine, the thickness of cartilage between humans and animals exists in all areas of the joints, and the thickness of the hyaline cartilage layer on the inner con of humans is 5-8 times that of adults. As we all know, the rabbit. Although there is no significant difference in the size of cartilage cells between human and rabbit articular cartilage, the matrix of a single cartilage cell in the human radial bone layer is 7-8 times that of rabbits. The anterior articular cartilage of the human femur is higher than the posterior articular cartilage. The situation of rabbits is just the opposite of that of humans; the different mechanical properties of cartilage lead to different repairs to cartilage after cartilage damage; differences in internal structure and mechanical properties will also affect the results of the study The evaluation of animal models can be carried out. You will see that it is difficult to apply the results to the human body, to repeat experimental results, or to apply the same experimental elements to different animal models and animal models with different results. Therefore, only with a comprehensive understanding of the biological and structural characteristics of human and animal articular cartilage can a more ideal experimental model be replicated.