动物模型 z-bo 2020-08-04 575

　　Lung syndromes are caused by strain, feeling of exogenous pathogens, chronic asthma and cough and injury to the lung, mainly including lung qi deficiency, lung yin deficiency, and lung guarding.

　　[Modeling mechanism] At present, the main method for establishing lung deficiency syndrome models in China is smoking (such as SO2, shavings tobacco, hydrocortisone, reserpine and thyroxine application plus shavings smoking, etc.), which first causes animal cough The syndrome of asthma develops into the syndrome of lung qi deficiency. Traditional Chinese medicine believes that chronic illness, cough and wheezing can damage the lung qi and cause lung qi deficiency. Therefore, the smoking model method meets the theoretical requirements of Chinese medicine.

　　[Method of Modeling] Using male NIH mice weighing 20-25g, the mice are placed in a static poisoning cabinet (35cm×50cm×50cm) every morning, smoked with cigarettes, 2 consecutively. Each cigarette was burned for about 10 minutes, and the interval between two cigarettes was 5 minutes. For 6 consecutive days a week, the test mice showed a poor attitude and slowly gained weight. They were sacrificed at the 4th, 6th, and 14th week for observation, and their bronchial inflammation was observed. The degree increases in turn.

　　【Features of the model】Model animals can have symptoms such as weak cough and pale red tongue, which are basically the same as clinical witnesses. In addition, it is also manifested as decreased activity, disheveled hair, listlessness, fatigue, decreased diet, and slower increase in body mass.

　　[Model evaluation and application] This type of model draws on the successful experience of Chinese and Western medicine in modeling. It is not only connected with the theory of Chinese medicine, but also consistent with certain diseases of modern medicine, and is easy to operate and repeatable. it is good. However, because the "syndrome" of Chinese medicine and the disease of Western medicine are two completely different concepts, it is difficult to unify in theory. Therefore, the theoretical basis of the current model cannot fully reflect the theoretical characteristics of traditional Chinese medicine. This model can be used to study the etiology, pathogenesis and drug treatment of lung deficiency syndrome.