动物造模,第三腰椎横突综合征动物模型 z-bio 2021-12-22 453

　　The third lumbar transverse process syndrome is a common disease in human clinical orthopedics. The disease is mainly caused by the posterior branch of the second lumbar vertebra passing through the dorsal side of the transverse process and descending into the spinous muscle. The surrounding muscle fibers, ligaments and other tissues attached to the transverse process are aseptic inflammation of the bone-muscle system. Adhesion and scars caused by injuries.

　　(1) Reproduction method Intramuscularly anesthetize 10 smianxin at a dose of 25 mg/kg body weight, inject the experimental rat (or rabbit), and cut the rat (or rabbit) longitudinally through 3 cm of the back under aseptic conditions. . , S vertebrae are bluntly dissected on both sides to expose the 3rd to 4th lumbar vertebrae (L3?L4) spinous processes, treat the surrounding tissues to the lamina with micro orthopedic instruments, and protect the tissue structure of the lumbar nerve. Intervertebral foramen: The penetrating part of the posterior branch of the deep fascia of the lumbar spine is completely separated. Next, cut out a gelatin sponge of about 0.5mm×0.5mm and transplant it under the middle layer of the deep fascia in the posterior half of the transverse process of the third lumbar vertebra. The vertebral fascia and skin were sutured equidistantly with a No. 3 needle and a No. 0 screw, and the incision was washed repeatedly with gentamicin.

　　(2) Model characteristics: In the early postoperative period, the animals showed obvious mechanical irritation and allergic behavior. The histopathological observation of the animal's third lumbar joint showed that the blood vessels were dilated, hyperemia and neurofibrillary edema 4 days after the operation. The inflammatory reaction in the muscle is very serious, and the reaction between connective tissue and fascia is very mild. There is no foreign body reaction around the material. Tissue congestion continued for 10 days after surgery, and the degree of inflammation of muscle and internal connective tissue was about the same. The inflammatory cells are dense, the material is destroyed, and most of it is absorbed by the cells. Thirty days after the operation, the material was completely absorbed, the inflammatory reaction of the muscle was slight, and the damage was repaired. Tendofascial cells aggregate, fibroblasts begin to proliferate, collagen remodeling begins, and the original scar formation can be seen. The results of the plasma test showed that in the early postoperative period, the levels of prostacyclin (related to the degree of hyperemia) and serotonin (which can cause inflammation at low concentrations) increased significantly.

　　(3) The transverse process of the third lumbar vertebra of medical staff has a special position and function in the lumbar spine, and the tensile stress at its end is greater than that of other lumbar vertebrae. If the lumbar spine is overloaded or the working position is not good for a long time, the third lumbar spine will be the longest and will receive the greatest force as a lever. All connected ligaments, muscles, fascia and tendons are also subject to the greatest pressure and are easily damaged. They are acute and chronic injuries and will affect the end tissues of the transverse process of the third lumbar vertebra, especially the invasion of blood vessels. the reason. It causes or aggravates the supply of local tissues, the pathological changes of the end tissues of the transverse process of the third lumbar vertebra.