动物造模,神经根型动物模型 z-bio 2022-01-05 325

　　Mechanical compression of nerve roots is not really directly related to the development of this disease. The inflammatory response, malnutrition and impaired conduction properties of nerve roots are equally important.

　　(1) Copy method Rats anesthetized by intraperitoneal injection of 30 mg/kg body weight of sodium pentobarbital, regularly prepare the skin, disinfect and drape, and mark the spinous process of the second thoracic vertebra. .. Make a longitudinal incision in the skin and subcutaneous tissue along the spinous process with the neck 7 as the center. The incision is about 1.5 cm. Use a sharp knife to separate the muscles on both sides of the spinous process so that both sides of the spinous process and lamina are exposed. Use eye scissors to cut out the lamina above the transverse processes on both sides. The spinal cord of the spinal canal is exposed at the 6th and 7th necks. Use peeling forceps to push the spinal cord to the right, exposing the 6 and 7 nerve roots on the left neck. Dip a certain amount of filter paper under the formaldehyde. Nerve roots 6 and 7 in the axilla. Carefully stop the bleeding, aseptically suture each layer and wrap the bandage.

　　(2) Three days after the modeling of the model characteristics, the nerve roots surrounding the inflammatory substances in the model group were mainly severe vasodilatation, congestion and nerve fiber edema, accompanied by a small amount of inflammatory cell response. Seven days after modeling, the nerve roots surrounding the inflammatory material in the model group were characterized by severe inflammatory cell infiltration. Accompanied by nerve fiber edema and degeneration; 14 days after the establishment of the model, the nerve root granuloma surrounding the inflammatory material in the model group was severely formed in the multinucleated cells. It was found that fibroblasts and collagen fibers were infiltrated with different degrees of inflammatory cells. Twenty-one days after modeling, the collagen fibers of the nerve roots surrounding the inflammatory substance in the model group increased, leading to severe scar formation.

　　The mouse is not active. If there are external factors, you can crawl slowly on the right upper and lower limbs. The left upper limb is close to the chest, not daring to touch the ground, the elbow and wrist joints are not moving, and the second and third toes are tightened (inflamed and swollen nerve roots, animal self-defense is considered to be avoided). At the same time, gently touch the seven nerve root distribution areas of the neck with a cotton swab, and the model mice immediately dodge. And keep screaming (this may be a sign of hyperalgesia). Experimental observations showed that the behavior of model mice began to improve within about 2 weeks. Recovery movement means that the left upper limb cannot touch the ground near the chest and the elbow joint touches the ground. Pain perception varies from hyperalgesia to insensitivity.

　　The amplitude of somatosensory evoked potential N11 in model rats decreases, N9? The incubation period of N13 is prolonged; the content of substance P in rat nerve roots (a measure of pain sensitivity) increases significantly in different postoperative periods. The severity of PGE2 expression (reflecting inflammation) is significantly increased in rat plasma.

　　(3) Comparative medicine radiculopathy Radiculopathy is mainly caused by cervical degeneration, and its root canal stenosis is accompanied by secondary physiological and biochemical changes, manifested as metabolic disorders and metabolites. Stimulate silence (protrusion of lumbar spinal stenosis-like substances, accumulation of acid products, etc.), release of biologically active substances (histamine, phospholipase A2, substance P, prostaglandins and other chemicals). Nerve roots are secondary to hyperemia, edema, inflammation and conduction disorders, among which chemical radiculitis may be an important pathological and physiological factor of the disease. Through pathology, behavior, electrophysiology and radioimmunoassay, experimental methods have been fully proven to cause the inflammatory response of cervical radiculitis in rats, which is related to the acute pathological symptoms of cervical radiculopathy.