动物造模,颈椎力学失衡动物模型 z-bio 2022-01-05 476

　　(1) Copy method After cutting and washing the rabbit neck, intramuscularly anesthetized with ketamine at a dose of 0.1 g/kg body weight; after adequate anesthesia, fix it on the operating table in a lying position. , Then spread out the towel, disinfect with iodine and ethanol, and cut the occipital bone from a 7-7 cm long midline longitudinal incision into the spinous process of the second thoracic vertebra through the OA ring. After an incision is made in the skin, the spinous process is completely exposed until the blunt spines are separated. Remove all the muscles attached to the spinous process lamina and small joints on both sides of the spine, and then remove the C1 to C7 supraspinous ligaments and interspinous ligaments in turn. After the operation, suture the subcutaneous fascia and skin in turn. The gauze is applied to the incision and will not be replaced after it falls off.

　　(2) X-ray model features: 8 months after the operation, there was obvious intervertebral stenosis, osteophyte formation on the anterior edge of the vertebral body and cervical spine correction. Histological examination: Three months after the operation, cracks appeared in the intervertebral disc ring, the position was slightly irregular, and the nucleus pulposus shrank or shrank. A few intervertebral discs showed mild disc herniation. Irregular hyperplasia and calcification are found in the cartilage endplate. Osteophytes are formed on the anterior edge of the vertebral body, and their cellular components are chondrocytes. Obviously, the cartilage endplate is elongated and proliferating. Eight months after the operation, the nucleus pulposus of the intervertebral disc was completely fibrotic, the layered structure of the annulus fibrosus disappeared, and vitreous disease was observed. Osteophytes are formed on the anterior edge of the vertebral body.

　　(3) Compared with medical models, this method will not damage the intervertebral disc, but will gradually cause cervical intervertebral disc degeneration. The animal bows because the soft tissue structure behind the animal’s neck is destroyed. This is in line with the trend of white-collar workers. An epidemiological study on the etiology of cervical spondylosis. The appearance of chondrocytes in the annulus is an important sign of intervertebral disc degeneration. Regarding the etiology and etiology of cervical spondylosis, it is generally believed that cervical spondylosis is the root cause of cervical spondylosis, and the mechanism of cervical spondylosis can be summarized as an increase in the activity of degrading enzymes in the intervertebral disc. The nutrient supply and the percentage of living cells are reduced. Reduce; post-translational protein modification and matrix fatigue and exhaustion. The intervertebral discs of all individuals undergo similar changes during their natural growth process, but the extent and time of the changes are different. Biomechanical imbalance will accelerate this change and exacerbate degradation.