动物造模,椎动脉型动物模型 z-bio 2021-03-30 338

　　(1) Replication method Inject 10ml tissue sclerosis agent 775 into the bone side of the 3rd to 5th cervical vertebra on the left side of the rabbit, repeat once every 2 weeks.

　　(2) After the model feature is modeled, starting from the 2nd and 4th weeks, the right vertebral artery (RVA) and basilar artery, left vertebral artery (LVA) and the average blood flow velocity decreased significantly, while the LVA decreased significantly. It is clear. After modeling, starting from the 4th week, the pulsation index, LVA resistance index and basilar artery resistance index increased significantly.

　　(3) The mechanism of vertebral artery cervical spondylosis in comparative medicine is complicated. It may be caused by the back side of the cervical disc (connected and fixed to the vertebral artery). It compresses the vertebral arteries and retracts the intervertebral discs. Gender changes can shorten the distance between the intervertebral space and the transverse process, causing movement disorders caused by vertebral artery strain and cervical imbalance, thereby affecting the cervical spine. The blood supply of the vertebral artery (VBA). In addition, the above-mentioned pathological changes stimulate cervical sympathetic nerve plexus or vascular disease, and anatomical changes are also the cause of abnormal vascular function of VBA.

　　At present, preparations for cerebral ischemia, including animal models of vertebral basilar artery insufficiency (VBI), mainly use surgical methods, but whether it is clamping the lower cerebral artery or compressing the labyrinth artery, the pathological mechanism of vertebral artery type cervical spondylosis, Especially the mechanical cause of this type of cervical spondylosis, regardless of whether the BA is ligated with silk or not. In this way, the drug was injected directly into the left side of the cervical spine and the muscle tissue near the rabbit, causing unilateral VA fibrosis, unilateral cervical fibrosis and contracture, so that the VBI of vertebral artery cervical spondylosis was successful, and the animal was successfully prepared. model. . The anatomy of the rabbit’s cervical spine and intracranial vessels showed that the rabbit’s skull base vessels have a complete cerebral artery circle (WillisCircle) and are similar to humans, but the rabbit’s cervical spine has vertebrae at the tip. Only an artery that passes through it without forming a side hole is formed. 775 solution can be injected into the "lateral groove" of the cervical spine and clinical sites to completely penetrate the vertebral artery and surrounding muscle tissue. The clinical mechanism of 775 injections is to shrink and fibrosis the varicose vein plexus. Pathological observation confirmed the model. The degeneration and necrosis of the VA tube wall and nearby muscle tissue occurred in the early stage of injection, and then gradually became fibrotic. By the 8th week, the artery wall and nearby muscles were severely fibrotic and contracted. The method can completely achieve the purpose of blocking VA blood.

　　Under normal circumstances, when humans turn their heads, one side of the vertebral artery will twist and narrow the lumen or completely occluded, while the other side of the vertebral artery will supplement the supply of the basilar artery. The contralateral vertebral artery is also affected, resulting in insufficient blood supply. The model created in this way showed that the neck muscles on the injection side were linear in the early stage, showing muscle spasm, fibrosis, and contracture, disrupting the mechanical balance mechanism of the cervical spine, and providing blood on the other side.