动物造模,犬腰椎椎管狭窄模型 z-bio 2021-12-23 553

　　(1) Breeding method After anesthetizing the experimental dog from the great saphenous vein with 3% pentobarbital sodium 30mg/kg body weight, perform tracheal intubation and shave the waist and fix it on the operating table. Inclined position and waist s cushion. High. Due to regular disinfection of the dural sac, L6? S1 midline posterior incision, skin, subcutaneous tissue and dorsal membrane cuts, spinal cord muscle detachment and complete hemostasis, L7 spinous process and lamina expansion, and bilateral L7 exposed nerve roots, polyester bone area (2.8 mm wide, 8.0 cm) Long) is made of polyester surgical repair material, and fixed at the height of the L7 vertebral body with arteriole clips and dural sacs (both sides) (including L7 nerve roots). The fixation method is as follows. The area of the spinal canal is reduced by about 50%.

　　(2) Model features After surgery, model animals may have mild or moderate dysfunction. This is manifested as a desire not to stand, walk or drag both hind limbs. After 3 weeks, you can climb a slope of 20°, but you will still experience slight resistance. After 1, 2, and 4 weeks, the latency of sensory and motor evoked potentials was significantly extended. Microhistopathological observations showed renal tubule hemorrhage, inflammatory cell infiltration, fibrous tissue proliferation, nerve swelling and degeneration, and partial vacuole degeneration of ganglion cells; it also indicated that cells and collagen had proliferated. When observed under an electron microscope, the large fibrous myelin sheath is very clearly swollen, some of which are onion skin-like changes. The axon is compressed and partially degenerated. Endoneural edema and hemorrhage, fibroblasts and collagen fibers multiply. , And the number of unmyelinated nerve fibers has increased.

　　(3) Comparative Medicine Human degenerative lumbar spinal stenosis involves one or more segments and can cause the cauda equina to be compressed into one or more segments. More than 90% of patients with intermittent lau line suffer from 2 or more central spine stenosis, or 1 central spine stenosis combined with nerve root canal stenosis. Different degrees of cervical spine stenosis of the dog's dural sac will compress the cauda equina and cause different degrees of lumbar spinal stenosis. As the degree of stenosis increases, sensory and motor function abnormalities gradually appear, the latency of evoked potentials is prolonged, and tissue damage is aggravated.