动物造模,大鼠腰椎椎管狭窄模型 z-bio 2021-03-30 257

　　(1) Reproduction method Anesthetize rats by intraperitoneal injection of 50 mg/kg body weight of phenobarbital. After anesthesia, the rats were fixed on the operating table for lumbar spinal canal surgery. Make a longitudinal incision from the third lumbar vertebra to the animal's ac bone, peel off the paraspinal muscles on both sides, and remove the fifth lumbar vertebral lamina and upper and lower joints. At the same time, the protrusions were removed, the lower articular process of the 4th lumbar vertebrae and the upper articular process of the 6th lumbar vertebra were removed, the right bone was collected, and then the excised lumbar lamina fragments were transplanted epidurally to avoid damage to it. . Horsetail. Raise animals regularly and eat freely.

　　(2) After 9 months of model feature modeling, the model animal's response to thermal stimuli was lower than before. Electrophysiological measurements showed that the SSEP of model animals at 8 or 9 months after surgery was significantly lower than that of normal animals. Compared with normal control animals, the average cross-sectional area of the fifth lumbar spinal canal of model animals has been reduced by nearly 50%. At the same time, the model animals have severe nerve root adhesion, obvious spinal canal stenosis, adhesion between cauda equina nerve fibers and adhesion to the dura; disappeared. The nerve fiber wall enlarges, the number of interstitial cysts increases, and the swelling is obvious.

　　(3) Comparative medicine This model uses extensive resection and bone grafting of the fifth lumbar vertebra to cause chronic progressive lumbar spinal stenosis, thereby establishing an animal model of lumbar spinal stenosis. Gait analysis showed that the gait significantly impaired the angle and distance of the bipedal movement, resulting in abnormal bipedal movement at the end of the gait. Compared with the control group, the walking distance was reduced by about 80%. After a few minutes of rest, walking returned to normal. These results are comparable to dyskinesias and intermittent lau routines caused by lumbar spinal stenosis. Depends on the response time to thermal stimuli to assess changes in sensory function. Compared with normal control animals, the reaction time of this model was reduced by 50% or more at 9 months after surgery, while the lower limbs of model rats showed hypersensitivity reactions. These changes are consistent with the chronic course of cauda equina syndrome. SSEP, as a means of evaluating pressure injury in cauda equina syndrome, has obvious clinical and experimental significance. In the animal model of the cauda equina compression experiment, the sensitivity of the cauda equina nerve is increased after compression. The cauda equina of model animals established in this way may have obvious conduction obstacles. In order to understand spinal stenosis and cauda equina nerve degeneration, a histopathological section of the fifth lumbar spinal canal was taken. Microscopic observation showed that no spinal stenosis was observed in the model animals after 1 month. There was no degeneration of the cauda equina nerve after the operation. Nine months after the operation, the average cross-sectional area of the spinal canal of the model animals was significantly reduced. The cauda equina nerve fiber is obviously degenerated. , And a series of pathological changes that are clinically very similar to human lumbar spinal stenosis.