动物造模,慢性实验性癫癎模型 z-bio 2021-04-01 318

　　(1) The commonly used method of reproduction is cats with males and females weighing 2.5 to 3 kg. The anesthesia and surgical procedures are the same as the ferrous sulfate model. Place the four cortical radio recording electrodes 10 mm before the coronal suture, 10 mm behind the coronal suture, and 3 mm left and right, and fix them with tooth powder. After the operation, kanamycin (125 mg/day per animal) or gentamicin (20,000 U/day per animal) was injected intramuscularly for 3 days to prevent infection. Experiment after a week. Penicillin sodium or potassium is injected into cats at a dose of 250,000-400,000 U/kg body weight after surgery. Penicillin can also be injected into different parts of the brain. Alternatively, it can be injected into a cotton swab containing 1.7-3.4 mmol/L penicillin. Next, observe the general activity of the animal and record the EEG and cortical waves at the same time. Rats and monkeys can also be used in experiments.

　　(2) Model characteristics 30-40 minutes after penicillin injection, the animal suffers from local and systemic clostridia. It manifests as clonic eyelid blinking, ear muscle spasm to generalized clonic spasm, and each episode lasts only a few seconds. At the beginning of the lesion area, during repetition and synchronization, spikes and wave discharges can also be recorded. Penicillin is a GABA receptor blocker, and its mechanism of action may be because penicillin binds to the GABA receptor and blocks the chloride channel that binds to the receptor, thereby blocking the postsynaptic depression caused by GABA.

　　(3) Using drugs, microinjection or intramuscular injection to compare large doses of penicillin with different parts of the brain can form a typical pathological model similar to clinical mild epilepsy. This model shows symptoms similar to mild clinical seizures and is paroxysmal. Symmetrical 3-7Hz synchronous high-voltage spikes, each burst discharge lasts for 1-3 seconds, this typical pico wave lasts for 4-5 hours, and then gradually weakens into a slow wave with low amplitude. This model is suitable for studying the spread of epileptic seizures and the neural basis of epilepsy.