Syndrome of deficiency of blood is caused by excessive blood loss; or weakness of the spleen and stomach, and insufficient biochemistry; or excessive seven emotions, dark consumption of yin and blood; or improper medication.

　　[Modeling mechanism] There are modeling methods such as tail-cutting blood loss method, drug injection hemolysis method, and radiation method. If drugs are used, the strong oxidant acetophenazine can disintegrate red blood cells, causing hemolytic anemia, and the TCM blood deficiency syndrome appears.

　　[Modeling method] Use 2% acetophenazine physiological saline solution, 1ml/100g, subcutaneous injection, can be administered once, or three times on the first, 4, and 7 days. One method halves the second and third doses.

　　【Features of the model】On the second day after the injection of the drug, the animals began to show clinical manifestations of blood deficiency syndromes such as pale lips or mucous membranes, weakness of limbs, and loss of hair.

　　[Model Evaluation and Application] The model is simple to replicate, suitable for large and small animals, with good repeatability, but short maintenance time. It can be used to confirm the quality of blood deficiency, the screening of treatment prescriptions and the research of mechanism.