动物造模,遗传性癫癎模型 z-bio 2021-11-10 277

　　(1) Copy method Sensitive gerbils are raised in separate cages, and they can eat and get drunk at will. In addition to general hygiene requirements, in order to avoid cramps, please keep quiet indoors, and do not make noises or move the cage when feeding or drinking. Measure the attack intensity once a week. When measuring, pick up the cage and shake it gently, or remove it from the cage and place it on the table to cause an attack. Record the extent of the attack. There is an incubation period of 3-5 days after the seizure, and the stimulation given at this time will not cause the seizure.

　　(2) Model features Sixth grade animal epileptic seizures: grade 0, no seizures, normal activity, grade 1, spasm of nose hair and blepharoplasty, stopped ear movement, grade 2. Can be divided into 2 levels. , Animal collapse, mild tonic-clonic seizures, sometimes generalized convulsions; grade 3, animal squatting in kangaroo posture, forearm clonic seizures and gripping contact; grade 4, unconscious tonic-clonic seizures Onset of generalized clonic seizures: 5. Several animals died of tonic-clonic seizures. The entire episode lasts 40 to 50 seconds. Design the dosing regimen according to the research needs, and observe and compare the intensity of epileptic seizures in animals.

　　(3) Comparative Medicine Mongolian gerbils are rodents belonging to the subfamily Mongolian gerbils and are a multi-purpose experimental animal. He is good at jumping and standing upright, and the incidence of spontaneous seizures is high, ranging from 20% to 30%. After several generations of inbreeding, selecting Mongolian gerbils that are sensitive to seizures for inbreeding and breeding provides a purebred hereditary epilepsy model. Seizures can be as high as 95%. It starts at 2 months after birth, reaches its peak at about 3 months, and lasts for a lifetime. The mechanism of this type of epilepsy is still unknown, because it is not clear which genes are mutated and are mainly genetically related.

　　The incubation period of 3-5 days after the attack and arrest may cause an attack and cause some problems for the research work. These shortcomings must be considered when designing the experiment. It is best to make the medicine into a natural oral solution, but the difference in the amount of water the animals drink will affect the correct judgment of the result.