动物造模,脊髓压迫损伤模型 z-bio 2021-12-09 965

　　Spinal cord compression is one of the important causes of spinal cord injury. The injury model mainly simulates spinal cord injury caused by spinal cord space-occupying lesions, which can be divided into acute compression injury and chronic compression injury. Compression is usually caused by transplantation of foreign bodies, such as expanded balloons, stainless steel screws, tumor cells, and silicone sheet grafts. The pathophysiological process of acute compression is similar to the spinal impingement model. Chronic spinal cord compression is a non-transient injury that helps to detect changes in nerve function and metabolism.

　　According to the method and duration of compression or compression, spinal cord compression can be divided into many types, including ventral and dorsal compression injury models, static compression and dynamic compression. Compressive force can be generated by compressing balloons, sacs, arterial clamps, weights, compression forceps and filling materials. The degree of compression damage mainly depends on the size of the pressure and compression time. Compression of the spinal cord, coupled with major mechanical effects, can lead to impaired blood supply and lead to degeneration and necrosis of spinal cord tissue. Tadoff et al. The resulting balloon compression method is to place an inflatable balloon between the vertebrae and the dura. After the animal has fully recovered after the operation, the balloon is inflated and inflated at various rates. Any part of the spinal cord may be damaged. The amount of gas injected and the compression time can be selected arbitrarily. This method is simple and reproducible, and has many similarities with intravertebral hemorrhage and spinal cord compression caused by space-occupying lesions that occupy spinal canal and vascular diseases in clinic. Usually used to compare shock and compression mechanisms. Spinal cord injury. After Tadov, Seijun et al. used a balloon catheter to transplant the epidural space through the intervertebral foramen to the L1 level and inject saline. Depending on the amount of saline injected, it may cause mild, moderate or severe spinal cord. Injured Tian Wei and others used a metal wire to insert a silicone sheet through the ligamentum flavum into the spinal canal and compress the spinal cord. This method reduces surgical trauma and simplifies the operation because it does not require opening the lamina. Not only can be accurately positioned, but also can be repeated operations. Good sex, lower mortality and more uniform patterns. A feature of these methods is that the transplantation of foreign bodies first causes acute compression and then continuous chronic compression. This is a clinical pathology of acute intervertebral disc herniation and prolapse leading to spinal cord compression, similar to a process.

　　The stainless steel screw compresses the spinal cord and causes damage. In 1990, Hashimoto applied it to the rat's T11 horizontal spinal cord, providing a practical and simple model of spinal cord injury caused by progressive spinal cord compression. Kong Kangmei of Henan Medical University improved the model by fixing a stainless steel screw compressor and a plexiglass plate on the back, thereby creating a progressive spinal cord compression model. This model can better simulate the natural process of chronic compression, but it will increase the number of operations and trauma.