动物造模,脊髓挫伤模型 z-bio 2021-04-01 382

　　Spinal cord contusion (contusion injury) was proposed by Allen by copying the spinal cord contusion model using weight loss methods. The specific method is to use a heavy hammer of specific mass to drop vertically along a series of tubes and hit a specific spine. Code snippet. In this method, the height of the weight, the weight of the weight, etc. are adjusted to adjust and control the drop impact force, and the spinal cord segment to be impacted is limited to reproduce various degrees and types of spinal cord contusion models.

　　Current research shows that this method has the following characteristics:

　　(1) Maintain the integrity of the dura mater, prevent exposure of the spinal cord and leakage of cerebrospinal fluid, while effectively preventing exogenous components from invading the injured area. This model is close to the pathophysiological characteristics and change rules of human spinal cord injury, and is of great help in studying the pathological changes, regeneration rules and interactions of neurons and glial cells after spinal cord injury, as well as neuroprotective strategies.

　　(2) This model is close to the pathophysiological characteristics and changes of human spinal cord injury. Early tissue edema, bleeding, and necrosis, followed by regular local repair and tissue reconstruction. Finally, chronic necrotizing cysts are formed, parenchymal cells atrophy and glial scars are formed. The temporal and spatial effects of tissue injury are very consistent with human spinal cord injury. Therefore, this model can be used to study the pathophysiological mechanism and experimental treatment of spinal cord injury. It is currently a model closely related to clinical spinal cord injury. However, due to the different types of animals used, the size of the same type of experimental animals is also different. It is difficult to determine the shock threshold that causes paraplegia in different animals. In addition, the instantaneous spinal cord and spinal cord deviation of heavy objects often leads to a contradiction between the result of the injury and the degree of the injury, and the difference caused by the same potential energy is usually very large. In order to reduce the experimental error caused by these uncertainties. Scholars at home and abroad have improved the handling methods of heavy objects to varying degrees and have come up with various improved animal models. current. Commonly used rodent spinal cord impact injury models are OSU impactor, IH impactor and MASCIS impactor (formerly NYU impactor). Through technological improvements, these impactors can monitor damage parameters such as impact velocity and tissue displacement, reduce the variety of damage, and further align the model with preset standards. There is a good correlation between the degree of injury, behavioral recovery and histopathological changes. The impact model simulates the injury caused by compression of the back of the spinal cord, but the clinical spinal cord contusion is more complicated. The pressure on the injured spinal cord may come from the front or back of the spinal cord. This is also one of the shortcomings of this model.

　　The bruise model is usually used to study the law of neuron regeneration after injury and to explore neuroprotective strategies. Ates et al. used a modified weight loss method to create a spinal cord injury model to study the neuroprotective effects of resveratrol. Through biochemical analysis, neurobehavioral and histopathological evaluation, we confirmed that resveratrol has an excellent therapeutic effect. YuMethylprednisolone is a medicine used to treat spinal cord injury. Vitellaro-Zuccarello et al. treated rats with spinal cord injury with recombinant human erythropoietin and found that the density of serotonergic plexus around motor neurons increased. This indicates that recombinant human erythropoietin can protect the white matter of the spinal cord by promoting white matter remyelination. Koopmans is the same as injecting a phosphodiesterase inhibitor and thalidomide into the abdominal cavity of depressed rats. As a result, the production of TNF-α and IL-1β was reduced, and after 6 weeks of treatment, the white matter lesions and motor function scores in the injury center were also obvious. Improve. The impact model 2 is used to improve the Mien Strike device. The weight of the device is electromagnetically controlled, so it only strikes the spinal cord once. By clamping the two ends of the injured spine, the spine is stabilized and affects the accuracy of the animal's breathing on the blow, thereby achieving the standardization and repeatability of the improved mouse spinal cord injury model.