动物造模,水平转盘睡眠剥夺法 z-bio 2021-04-01 475

　　(1) The copy method experiment equipment consists of a computer console, a horizontal turntable and two open rectangular organic glass cylinders. The diameter of the turntable is 46 cm, which can be randomly rotated clockwise and counterclockwise under computer control. The dimensions of the two glass cylinders are 60 cm x 20.5 cm x 60 cm, one of which is on the side wall of the cylinder. The bottom of the cylinder. The gap allows half of the turntable to extend from the gap into the two glass cylinders and rotate freely. The water is kept about 2 cm deep at the bottom of the tank, and the turntable is about 3 cm from the water surface. One week before the experiment, the microelectrodes were implanted into the head and neck of sleep-deprived rats, and the microelectrodes were connected to the computer console and placed on the turntable for 1 week, about 1 hour a day, to adapt to the environment. And allow mice to get used to the turntable. Activities, fluid intake, food, etc. During the experiment, the rats in the experimental group and the rats in the control group were placed on a two-cylinder turntable. When the computer monitors the brain electrical signals of the rats in the experimental group, they enter slow or fast waves. Fall asleep through the microelectrodes and immediately instruct the turntable to rotate for 6 seconds (rotate 1/3 within 6 seconds). If the mouse is aimed at the wall of the glass bottle, it will be blocked by water and may fall into the water. Glass cylindrical wall. Each time a sleep deprived rat in the experimental group fell asleep, the turntable would rotate in a random direction for 6 seconds. As the turntable rotates, both mice move passively with the turntable. Using this method, only one mouse was deprived of sleep at a time. When the deprived rats in the experimental group moved, drank or consumed water, the turntable would not rotate, but the rats in the control group could fall asleep. Make up for poverty. go to bed.

　　(2) Model characteristics: Since the sleep deprivation group and the control group are very similar, this method can reduce the stress response caused by different experimental conditions. You can try using TSD and SSD.