动物造模,脊髓损伤模型 z-bio 2021-04-01 465

　　Some scholars incorporate this method into the spinal cord compression model. Because it can be decompressed after compression, it is proposed separately. Rivlin et al. used a modified aneurysm clamp to clamp the spinal cord directly, which can reflect the relationship between different clamping forces, different compression times and the degree of spinal cord injury. Sheng et al. prepared a minimally invasive rat spinal cord injury model by incising the interspinous ligament to expose the epidural space without laminectomy, and placing a 15mm silicone tube perpendicular to the longitudinal axis of the spinal cord in the epidural space at the level of T11; Before placing the silicone tube, use a suture to pass through the silicone tube; remove the silicone tube at 1 min, 30 min, 60 min, and 120 min after spinal cord compression. Nervous system function was evaluated on 1d, 3d, 7d and 14d after injury and histological analysis was performed. BBB motor scores gradually deteriorated over time. Histological analysis showed that normal neurons in the ventral horn gradually decreased, and the cross-sectional area of cell necrosis increased. Later, Li Gang and others used aneurysm clips to establish a model. The type that causes human spinal cord injury in daily life has an important role in studying the pathophysiological changes in the acute phase after spinal cord injury, the timing of releasing clamp compression, electrical stimulation and neuroprotective intervention. The key to making this model is the determination of the clamp position and pressure. Such models are mostly closed injuries and non-transient injuries. They have both primary injuries caused by mechanical compression and secondary injuries caused by compression, which are similar to clinical ones. Facilitate the research and detection of neurological function and metabolic changes.

　　This type of model is mostly non-instant closed injury, which is convenient for the detection of nerve function and metabolic changes. It can simulate the natural injury process in a graded and quantitative manner, which is helpful to understand the proliferation of glial scars, and inhibits adverse pathological reactions after injury. The angle of promoting nerve regeneration. Explore ways to treat chronic injuries.