动物造模,急性运动性疲劳动物模型 z-bio 2021-10-26 321

　　1. Fatigue animal model induced by high-intensity exercise

　　Use healthy male SD rats weighing 250-350 g. (1) The replication method uses subjective observation indicators (changes in the general condition of rats, changes in running posture, changes in sports performance) to determine whether the animal feels fatigue and fatigue, and to judge the degree of feeling. The experimental rats performed horizontal running exercise at a speed of 20 m/min (based on BEDFORD's maximum oxygen uptake, which is more than 90% of the maximum oxygen uptake of high-intensity exercise) for a duration of 20 minutes. Give during exercise. Sound and brush stimulation. In the first 10 minutes, experimental rats can basically maintain their original working intensity without being stimulated. In the next 10 minutes of exercise, the pedal posture of the experimental rats was not so active at the beginning of the exercise, and the intensity was not so strong, indicating that their performance and fatigue were gradually decreasing. (2) Model characteristics The model established by this method induces physical fatigue faster due to its higher exercise intensity, but a high-intensity and short-term fatigue is relatively light.

　　2. Animal model of fatigue caused by aerobic exercise

　　(1) The replication method uses subjective observation indicators (same as above) to determine whether the animal feels tired and the degree of fatigue. The experimental rats performed a moderate-intensity horizontal running exercise at a speed of 18 m/min for a duration of 100 minutes. After long-term and moderate-intensity exercise, the rats showed colder expressions and slower reactions. Escape response

　　Avoidance is weaker than before exercise. In the later movement, the rats run much more difficult than before, especially in the last 20 minutes, when most of the rats' running posture changed from the first leg on the ground to half running. -Prone position Since the abdomen runs in a prone position in contact with the track, as the exercise time increases, the exercise capacity of the rat gradually decreases.

　　(2) Model characteristics After moderate long-term exercise, rats show obvious discomfort, which is more severe than the discomfort caused by a single high-intensity short-term exercise.