动物造模,侧向液压冲击脑损伤模型 z-bio 2022-01-07 480

　　The lateral hydraulic shock brain injury model quickly injects a certain amount of saline into the cranial cavity, which causes the deformation and displacement of the brain tissue, which leads to TBI. The hydraulic system consists of a circular liquid column (length 64 cm, diameter 4.5 cm), strike frame, oscilloscope and pressure sensor. One end of the circular fluid column is connected to the piston, and the other end is connected to the blow tube and the pressure sensor. In the event of an injury, the entire pipeline system will be filled with 37°C saline and closed, and the pressure generated after the hammer hits the piston will be transmitted to the cranial cavity through the fluid in the blowpipe and act on the tissue.

　　The specific steps are as follows: (1) Feed the rats 3 days before the operation, and fast for 8 hours before the operation. During the experiment, 3.6% chloral hydrate was injected intraperitoneally at a dose of 1 ml per 100 g and fixed in the prone position of the stereotaxic device holder.

　　(2) Cut 3 mm behind the left coronal suture and right coronal suture, and cut the first layer of skin 2 mm beside the middle sagittal suture. Drill the skull with a dental drill. The diameter of the bone window is approximately 3 milliliters. Fix the sterile blow tube on the bone window with self-curing denture powder.

　　(3) Open the bone window 3 mm behind the contralateral coronal suture and 2 mm next to the sagittal suture. The diameter is about 15 mm. Insert a 15mm silicone tube. Monitor intracranial pressure. Monitor blood pressure, heart rate, and breath of rats at the same time. Please wait.

　　(4) Establish a rat model of progressive head injury. Different blows are given according to the grouping situation (the size of the blow is determined based on preliminary experiments): 1313 to 3313 kPa or less for the mild injury group, 3314 to 5017 kPa for the elderly injury group, and 7314 kPa for the severe injury group ; The control group is unaffected, but the procedure is exactly the same.

　　The destructive power of the model is quantitative and accurate, and can directly reflect the pressure on the brain tissue. It has high reproducibility and can cause severe mild, moderate and severe head injuries. At present, this model has been widely used in neuropathology, neurobiochemistry, neuroelectrophysiology, neurotransmitters and receptors, neurobehavioral function, brain energy metabolism, cerebral blood flow, and drug efficacy after head trauma. However, the damage mechanism of this model is inconsistent with the man-made damage mechanism, the equipment is complicated and expensive, and it is basically developed and assembled in-house in China.