动物造模,记忆巩固障碍模型 z-bio 2021-04-02 410

　　1. Modeling material animal: healthy Kunming mice; drug: cycloheximide; equipment: MR-921 mouse jumping platform experiment automatic recorder.

　　2. The modeling method is to perform platform jump training first, and then immediately after training or 10 minutes before training, intraperitoneal injection of cycloheximide 120-150 mg/kg. The next day, we will test your memory score.

　　Jumping platform method: first put the mouse on the platform jumper, adapt to the environment for 5 minutes, and then gently place it on the platform. When the animal jumped off the platform and touched the copper grid with its limbs, it was stimulated by a voltage of 40V AC, and the escape of the mouse was recorded. Record the number of electric shocks (number of errors) within 5 minutes until the platform incubation period (EL), and use it as an academic record. .. The test is carried out after 24 hours. Place the mouse on the platform, record and evaluate the incubation period (SDL) of the mouse retained on the safe platform and the number of electric shocks (error) within 3 minutes. Memory capacity. If the mouse stays on the platform for more than 3 minutes during the test, its incubation period is 180 seconds.

　　3. Principles of Modeling Cycloheximide is a protein synthesis inhibitor that will adversely affect memory integration.

　　4. In the general post-modeling change and platform jump experiments, the number of errors in the training process of the normal control group was (2.82±1.35) times higher than that of the control group animals, and the test was carried out. The error is (0.34±0.57) times, the training (EL) waiting time is (14.0±4.9) seconds, the test (SDL) waiting time is (150.8±47.5) seconds, and the number of errors during the model group training is (2.19). ±1.23) times, the number of errors during the test is (2.08±1.45) times, the training waiting time is (13.3±5.0) seconds, the test waiting time is (51.7±20.3) seconds, and a memory integration failure has occurred, which indicates.