动物造模,口蹄疫病毒动物模型 z-bio 2022-01-07 438

　　(1) Replication method Use 0.01 mol/L PBS (pH 7.4) to dilute the foot-and-mouth disease seed virus ORMF8 to LD50 to 10-3 to 10-7/0.2 ml, and then titrate each titer of virus solution dilution. The guinea pigs were injected intracutaneously into the toes of the hind limbs at a dose of 0.2ml each.

　　(2) Model characteristics Guinea pigs infected with foot-and-mouth disease virus can cause typical diseases. This is the key to the successful establishment of a guinea pig foot-and-mouth disease virus infection model. Previous studies have shown that guinea pigs are long-term experimental animals used for foot-and-mouth disease infection. Infant mice among newborns are very sensitive to FMD virus, but less sensitive to FMD virus. 5 days after birth. In addition, adult mice and hamsters are also susceptible to FMD virus, but in guinea pigs, the disease does not seem to have a regular pattern. Guinea pig foot-and-mouth disease virus infection can lead to its typical disease characteristics and may lead to obvious lesions. Diseased guinea pigs will grow small blisters at the site of inoculation, which will be absorbed and disappear in the future without an eroded surface. However, it can be found on the limbs and toes of guinea pigs. Or, secondary blisters can form in the mouth, thinning the guinea pig's body and causing some deaths.

　　(3) Comparative Medicine All the original inactivated foot-and-mouth disease vaccines in my country use guinea pigs (or pigs and cattle) for efficacy testing. The World Organization for Animal Health (OIE) advocates the use of guinea pigs for vaccine efficacy testing, but it also recommends the use of parallel substitutes as routine testing methods. The large-scale use of guinea pigs for vaccine testing is expensive, long-term and labor-intensive. In order to obtain sufficient and reliable test data, the use of non-animal methods for testing is consistent with the development trend of the miniaturization of test organisms and non-animal testing. Guinea pig is a model animal that has been used for artificial infection with FMD virus for many years. It can be used not only to detect antibodies, but also to assess the immunity of FMD. Foot-and-mouth disease vaccine based on clinical symptoms and pathological observations.