动物造模,记忆巩固障碍模型 z-bio 2021-04-02 733

　　1. Modeling materials Animals: male and female, 18-20g, 4 weeks old Kunming mice; drug: sodium nitrite; equipment: XT-911 mouse jumping platform adjustment tester.

　　2. Modeling method: The XT-911 mouse platform controls the voltage of the conditioned reflex tester to 36V. During training, 4 mice in each batch were placed in 4 grids on the platform and adapted to the environment for 3 minutes. After energizing, most mice jump on the platform to avoid electric shock. When beating, when both feet touch the copper grid at the same time, the mouse will receive an electric shock. This is considered a wrong reaction. After 5 minutes of training, the test will be repeated after 24 hours. During the test, hover the mouse on the jump platform and start timing at the same time. Record the time when the mouse moves for the first time. This is the electric shock latency (ie, the error latency) and the number of jumps in it. 5 minutes (that is, the number of errors). As an observation indicator. After training, the control group was subcutaneously injected with the same amount of normal saline and 120 mg/kg sodium nitrite was subcutaneously injected into the model, and the study was conducted 24 hours later.

　　3. Modeling principle Sodium nitrite can cause memory integration disorder in mice.

　　4. After the model was established, the number of errors in the control group was (0.58±0.79) times, the incubation period was (249.8±85.8) seconds, the number of errors in model establishment was (4.08±1.73) times, and the incubation period was (27.5). ±4.3) seconds. Compared with the control group, the incubation period of the model group was significantly shortened, and the number of errors within 5 minutes was significantly increased.