动物造模,记忆巩固障碍模型 z-bio 2021-12-30 405

　　1. Modeling material animal: Wistar rat, body weight (216±10) g; equipment: electroconvulsive instrument.

　　2. The modeling method is to perform platform jump training first. After animal training, electrodes are connected to the head and nose to transmit electrical current. The maximum electroconvulsive treatment was performed with a stimulation intensity of 150V, an electric current of 0.1A, and a time of 0.3 seconds. After that, the stiffness of the limbs was used as a shock index, and the memory was measured when the shock was 30 minutes. ..

　　3. Modeling principle Electric shock will cause memory integration failure.

　　4. After the model is established, a water maze test will be performed 24 hours after the general change. The animal exploration platform latency (exploration platform waiting time, SPL) is (20±8) seconds after the electric shock, 30 minutes (55±17) seconds after the electric shock, and 24 hours (29±10) seconds; the number of times the animal is removed from the water The percentage of the corresponding position that passed through the original underwater platform within 120 seconds after the platform was dropped is the percentage of the total number of times the corresponding position of the platform passed through each quadrilateral (percentage between platforms, percentage between platforms), CPP) is (58 before electric shock ± 4) %, (27±5)% after 30 minutes of electric shock, (34±3)% after 24 hours. The results showed that SPL prolonged, CPP decreased, and the ability of forgetfulness and spatial positioning decreased to varying degrees after electric shock.