动物造模,急性肾功能衰竭 z-bio 2021-11-03 667

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　　(1) Acute renal failure model in which the dog’s renal artery is clamped: In the kidney, a 10 to 20 kg male dog is intravenously injected with 30 mg/kg sodium pentobarbital to anesthetize the left side. Clamp the artery for 1 hour, then relax, reperfuse the blood flow, and collect urine from the lateral ureter for phenotypic analysis of acute renal failure.

　　(2) Rat model of acute renal failure induced by surgery: SD male rats, weighing 280-350 g, anesthetized by pentobarbital sodium 50 mg/kg, cut both sides of the abdomen, removed the right kidney, and separated the left kidney stem. Clamp the left renal artery with a blood vessel clip for 60 minutes, and then suture the wound to resurrect the animal. Blood samples are collected daily to measure blood creatinine and analyze the phenotype of acute renal failure.

　　(3) Acute renal failure model with norepinephrine for domestic dogs: collect a male dog of 10-20 kg, inject 30 mg/kg of sodium pentobarbital intravenously, anesthetize, and insert the catheter into the urinary tract Urine norepinephrine was collected from one side of the patient and injected through the other renal artery at a rate of 0.75 mg/min. After 40 minutes, the drug was stopped and the phenotype analysis of acute renal failure could be performed within 1-4 hours.

　　(4) Rat hemoglobinuria acute renal failure model: Collect male rats weighing 150-250g, place them in a metabolic cage, record normal drinking water and urine volume, and keep water for 24 hours before administration. Lighter mice. In rats, 50% glycerol was injected into the muscles of the hind limbs of the rats at a dose of 10 ml/kg. After the injection, the rats can eat and drink freely. Proteinuria appears after 2 hours, and a stable model is formed after 48 hours. The experiment can be completed within 2-5 days.

　　(5) Acute renal failure model caused by glycerol: Collect male rats weighing 150-250 g and inject them intramuscularly with 10 ml/kg of 50% glycerol. After 24 hours, he developed hematuria, increased urea nitrogen, oliguria or anuria, and a similar acute kidney. failure. [Result analysis] Regular urine, serum creatinine, serum total protein albumin, serum urea nitrogen, serum sodium potassium and renal pathological examinations were performed. Renal failure in a canine model of acute renal failure with norepinephrine is reversible, and a delay of 2 hours of infusion can cause extensive renal parenchymal necrosis and renal tubular necrosis. If the blood flow is blocked for more than 4 hours, the rat will die of uremia. This model can be used to observe the effects of drugs on acute renal failure.