动物造模,旋转及震颤模型 z-bio 2021-12-01 567

　　1. Rotate horizontally

　　induced rabbit nystagmus model 1. Modeling material animal: rabbit; equipment: rabbit box, JKY-11 electric swivel chair, Rm-600 08-channel physiological recorder.

　　2. Modeling method Put the awakened rabbit in the rabbit box to make it quiet, put the rabbit box on the JKY-11 electric swivel chair, and insert two No. 5 stainless steel acupuncture needles into the inner and outer corners of the eyes. do. Approximately 0.5 cm is left under the skin of the left eye, and the needle penetration depth as a guide electrode is approximately 0.5 to 1.0 cm. The nystagmus signal is input to the physiological recorder through the guiding electrode and monitored by the oscilloscope. The nystagmus waveform is tracked by the tracer and recorded on the tape for backup. Fix the rabbit's head so that the line between the inner and outer corners of the eye and the horizontal line is about 30°, so that the horizontal semicircular tube can be stimulated most when the angular acceleration moves. Blindfold and stand for 15 minutes, then start the electric swivel chair.

　　3. Modeling principle Horizontal rotation will cause rabbit nystagmus.

　　4. Changes occur after modeling. The angular acceleration of the electric swivel chair is 15°/s2, the acceleration time is 8s, and the peak angular velocity is 120°/s. The rabbit's nasal cavity rotates. It rotates at a constant speed for 52 seconds and stops (stops within 0.5 seconds). Rotating rabbits can cause nystagmus.

　　2. Spontaneous nystagmus model after one side of the maze is destroyed

　　1. Modeling material animal: rabbit; drug: pentobarbital, alcohol; equipment: operating microscope, LMS-2B 2-channel physiological recorder.

　　2. Modeling method Anesthetize experimental animals with 0.12 mol/L pentobarbital (30 mg/kg) ear margin injection, make an incision behind an appendage of the tympanic membrane, cut the skin to the periosteum, and then open the belt The bones of the ear canal. After exposure, the tympanic membrane passes through the tympanic membrane, exposing the middle ear cavity. The round window membrane and the bone can be seen under the operating microscope, and then the bone is removed. It extends to the oval window, destroys and removes balloons and sacs, and absorbs membrane fragments. Use a microscope curette and a miniature electric drill to open the oval window and crochet it at a right angle to the vestibule. Rotate the crochet needle to destroy the upper, lower and outer parts, and then repeatedly clean the inner ear with 95% (V/V) alcohol on the ampla epithelium in the posterior semicircular tube to achieve complete destruction. Do it. Maze receptors. At this time, you can see that the rabbit has obvious persistent nystagmus, the affected side is in front, the healthy side is shaking, and the head is tilted to the affected side. The cavity is filled with iodoform gauze and an incision. The recording began approximately 14 hours after the nystagmus surgery.

　　3. Modeling principle One side of the maze is destroyed, causing spontaneous nystagmus in animals.

　　4. Changes after modeling After the operation, the rabbit had persistent and obvious nystagmus.

　　5. Precautions Strictly disinfect surgical instruments to prevent surgical infection, surgical trauma should be as small as possible, and strict aseptic operations should be performed. In the breeding room, keep the best temperature and humidity for the animals as much as possible.

　　3. Drug-induced muscle tremor model (1) Arecoline-induced tremor model

　　1. Modeling material Animal: mouse, weighing 18-25 g, or rat (body weight 180-220 g); drug: arecoline.

　　2. Modeling method Injecting 25 mg/kg arecoline into the abdominal cavity of mice or injecting 25 mg/kg arecoline into the abdominal cavity of rats will cause high-frequency tremor (13-22 Hz).

　　3. Modeling principle Arecoline can cause animal tremor.

　　4. Changes after modeling. The incubation period after modeling is 5 minutes, and the tremor lasts for 30 minutes. It manifests as tremors, curls, erect tails, salivation, lacerations, bowel movements and other parasympathetic symptoms.

　　(2) Tremorin-induced tremor model

　　1. Modeling material Animal: adult mouse; drug: oxyfibrillin, fibrillin.

　　2. Modeling method The mice were injected intraperitoneally with 0.14 mg/kg of oxidative tremor. Alternatively, intravenous injection of tremorin into mice will cause the tremor-induced tremor ED50 of 10-20 mg/kg.

　　3. Modeling principle Tremor must first be transformed into oxidative tremor in the body before it can take effect, and oxidative tremor can cause animal tremor.

　　4. Changes after model construction After injection of oxidative tremor, the incubation period is 5 minutes, and the tremor lasts about 30 minutes. After injection of trehalose protein, the incubation time is 5-10 minutes.

　　(3) Nicotine-pilocarpine induced tremor model

　　1. Modeling materials Animals: adult mice; drugs: pilocarpine, nicotine.

　　2. Modeling method 50 mg/kg pilocarpine was injected intraperitoneally in mice, and 2.5 mg/kg nicotine was injected intraperitoneally 20 minutes later.

　　3. Modeling principle Nicotine and pilocarpine cause animal tremor.

　　4. 10 minutes after nicotine injection, changes may occur after modeling. Head, body and tail tremors are mixed together, severe head shaking and retreat, salivation, lacerations, etc., and parasympathetic nerve excitement may also occur. . (4) The tremor model caused by Lycepine

　　1. Modeling material Animal: rat, weighing 200-300 g; drug: reserpine.

　　2. Modeling method Rats are injected subcutaneously with 1 mg/kg reserpine every day for 8-10 days.

　　3. Modeling principle Risepin can cause animal tremor.

　　4. Changes after modeling After modeling, the eyelids droop, the tail and limbs tremble, the tail tension increases, the body is stiff, bends backward, the front ankle joints are bent closer to the trunk, and the gait is abnormal. The above symptoms, such as slow movements, hair loss and A decrease in body temperature indicates the three basic symptoms of tremor and paralysis: tremor, limb tremor, and difficulty in movement. (5) The tremor model induced by physostigmine

　　1. Modeling material Animal: rat, weighing 200-300 g; drug: physostigmine. 2. Modeling method Intraperitoneal injection of physostigmine 0.5 mg/kg to model rats. The control group was injected with the same amount of saline.

　　3. The modeling principle of physostigmine can cause animal tremor.

　　4. Changes after modeling The incubation period after injection is 5 minutes, and the tremor lasts for 15-30 minutes.