动物造模,疱疹病毒,转基因动物模型 z-bio 2022-01-10 501

　　(1) Replication method Construct a nervous system stellate cell-specific expression vector containing human herpes virus 6BU83 gene, microinject it into fertilized mouse egg cells, and then construct a transgenic mouse strain expressing human herpes virus 6BU83.

　　(2) Model characteristics This model is a nucleic acid fragment specifically expressed in astrocytes of the mouse central nervous system. The first part of this fragment is the glial fibrillary acidic protein promoter, which can lead to good and specific expression in central nervous system astrocytes. Finally, there is a fragment of the mouse protamine 1 gene, which provides introns and polyA sites. The HHV-6BU83 nucleic acid fragment is inserted into the multiple cloning site between the two. Astrocytes are one of the main cell groups that make up the central nervous system of vertebrates, and are related to various central nervous system functions, such as signal transduction and energy metabolism.

　　(3) Comparative medicine The establishment of transgenic mice expressing HHV virus protein is very important for studying the relationship between HHV infection and related diseases. For some unexplainable diseases, the observation of genetically modified mice may be directly important. Related reasons. The establishment of mice expressing HHV gene specifically provides a meaningful animal model for further research on the relationship between HHV and diseases, reveals the pathogenicity of HHV, and explores the mechanism of specific diseases with unknown causes.