动物造模,兔肺炎衣原体肺炎模型 z-bio 2021-12-15 270

　　(1) Breeding method First take out a 1-month-old New Zealand rabbit, hyperventilate under mild halothane anesthesia, and then inoculate 1.0×10000000?5.0×10000000 CFU/ml pure chlamydia pneumonia from its turbinate. With plastic hose. 1.0 ml medium. The rabbits developed pneumonia symptoms on the 7th day, the symptoms began to improve on the 21st day, and the lungs looked completely normal on the 28th day.

　　(2) Model features Clinically, humans infected with Chlamydia pneumoniae may show self-limiting respiratory symptoms or no clinical symptoms. Rabbit pneumonia replicated in this model only infects a small number of pathogens through the nasal route, and model animals can develop symptoms of pneumonia. At the same time, the symptoms are milder and shorter than the mouse chlamydia pneumonia model. .. Performance is close to the characteristics of human Chlamydia pneumoniae pneumonia.

　　(3) From the 7th day of comparative medical infection, IgG antibodies can be produced in model animals. In addition to the presence of Chlamydia pneumoniae in the lungs and spleen of animals, this pathogen also exists in the liver and arterial arch. Some model animals may show early symptoms of arteriosclerosis. It has been shown that the model established in this way is not only a suitable animal model for studying chlamydial pneumonia, but also an ideal model for studying arteriosclerosis caused by chlamydial pneumonia.