动物造模,肺炎支原体模型 z-bio 2022-05-10 313

　　(1) Replication method The Mycoplasma pneumoniae strain (ATCC29342) was cultured in SP-4 liquid medium at 37°C for 72 hours. After centrifugation, the precipitate was prepared to 1×100000000 CFU/ml by SP-4 liquid culture. Two-month-old BALB/c mice were anesthetized by inhalation of methoxyhalothane, and over inhaled, 50μl of SP-4 liquid medium containing 1×100000000 CFU/ml Mycoplasma pneumonia was injected intranasally, and it was found that the mice developed depression1 -2 days after vaccination

　　(2) Model characteristics The development of Mycoplasma pneumoniae infection is related to the attachment and respiration of Mycoplasma pneumoniae. After attaching to vascular epithelial cells, host cells die and fall off, causing a series of weakened immune responses that trigger mild upper respiratory tract infections, severe fatal pneumonia, and a variety of other symptoms. Some scholars contracted mycoplasma pneumonia from the nasal cavity, causing lung infections in rats. The route of transmission is similar to natural human infection. This model is caused by the pathogenic mechanism and infection of Mycoplasma murine pneumonia. It is an immunopathological model because mice are easy to operate, have easy-to-obtain immunological reagents, and have Abundant immunological data are available for reference.

　　(3) Comparative medicine Mycoplasma pneumoniae airway infections are mainly pharyngitis, tonsillitis and bronchial infection, which are manifested as inflammation, accounting for only 3%. 10% of the cases showed pneumonia. The lung lesions of the model animals were obvious, the entire lung was congested, and the scattered necrotic lesions were of different sizes. The clinical symptoms caused by the infection of mycoplasma pneumonia in mice were also mild. Pathological anatomy showed inflammatory exudation. In the bronchi, trachea and blood vessels, solid pneumonia appeared, tracheal exudates, many of which were neutral and simple; the inflamed nuclear cells became inflamed; the inflammatory response began to subside on the 21st. In human cases of Mycoplasma pneumoniae infection, the pathogen has recovered from the disease. This situation may also occur in this model several months after being found in the airway. In the tracheal lavage fluid of model animals, TNF-α, IF . -γ, IL-6, IL-8, etc. increase significantly, indicating that the body is in a higher immune response state.