How to prepare an animal model of spleen deficiency syndrome with polymyositis (muscle arthritis)?
多发性肌炎,动物模型
z-bo
2020-08-05
547
Polymyositis (polymyositis) is an idiopathic striated muscle inflammation related to autoimmunity, which belongs to the category of TCM muscle paralysis. Because the spleen controls the muscles and is the source of qi and blood, spleen deficiency is the basic syndrome of the disease.
【Modeling mechanism】Using rabbit skeletal muscle homogenate plus Freund's adjuvant combined with hunger and satiety to form a polymyositis model of spleen deficiency syndrome, observe the changes in general conditions, muscle enzymes, muscle and lung tissue pathology.
【Modeling method】Experimental animal: Guinea pig. The guinea pig’s back was subcutaneously injected once a week, with a dose of 0.2ml of muscle homogenate+0.1ml of complete Freund’s adjuvant for a total of 8 weeks. With one-day fasting, two-day fat diet with grass. Observation indicators: muscle enzyme spectrum, creatine creatase, creatine phosphatase, lactate dehydrogenase, aspartate aminotransferase, guinea pig muscle disease examination.
[Model Features] After 4 to 5 weeks, guinea pigs will experience obvious fatigue, loose stools, decreased appetite, loose and unkempt coat, squint eyes, low activity and slowness, slow growth, weight loss, etc. Symmetrical muscle weakness of the extremities, difficulty in standing in heavy patients, and slow progressive progress. The muscle enzyme spectrum showed a significant upward trend, and the muscle pathology showed obvious changes in degeneration, atrophy and inflammation. And 20% to 30% have interstitial pneumonia of varying degrees.
[Model evaluation and application] This model uses rabbit skeletal muscle homogenate plus Freund’s adjuvant combined with hunger and satiety to form a polymyositis spleen-deficiency syndrome model, from the muscle enzyme spectrum, creatine creatinase, creatine phosphatase, and lactate The results of various indicators of hydrogenase, aspartate aminotransferase, and guinea pig muscle disease examination, especially the appearance of some guinea pig interstitial pneumonia, suggest that the immune method combined with the hunger and satiety method is basically successful. However, due to the high mortality rate, how to choose a better compound model remains to be studied. This model can be used to study the pathophysiology of polymyositis in Chinese medicine.
