动物造模,大鼠肺炎支原体模型 z-bio 2023-02-02 544

　　(1) Replication method Inoculate the international standard Mycoplasma pneumoniae FH strain (ATCC15531) into the mycoplasma liquid medium (the main components are bovine brain heart injection, sterile fetal bovine serum, fresh yeast injection, glucose, penicillin and phenol base Culture medium). Adjust the pH to about 7.6, for example red). Using the 10-fold dilution method, the test bacterial solution in the culture medium is diluted to a series of concentrations from 1x10-1 to 1x10-12. After 7 days of incubation at a constant temperature of 37°C, the medium changed from red to red. The largest dilution of yellow is used as the color change unit (CCU), and the concentration of the bacterial solution is calculated as CCU/ml. Raise experimental rats weighing 180-200 g, observe for 1 week, and then raise them in separate cages under standard conditions. Slowly inject 1000000 CCU/ml Mycoplasma pneumoniae medium into the nasal cavity, and use its natural inhalation effect to inhale 0.2 ml of bacterial solution from the respiratory tract to the lungs once a day. Observe their activities and diet every day after vaccination.

　　(2) Model characteristics: Three days after infection with Mycoplasma pneumoniae, model rats may experience depression, decreased activity, rough fur, elevated body temperature and obvious pulmonary damp sound. On the 10th day, the clinical symptoms worsened and purulent discharge appeared, especially in the nose. Individual animals had reduced red eyes, nasal congestion, and wheezing in breathing. Some animals will die within 10 days of infection. Ten days after the infection of the model animals, lung lesions were evident, indicating that the entire lungs were congested and bleeding. In severe cases, the lung lobes are scattered among necrotic lesions of various sizes. Histopathology was observed. Under the microscope, you can see obvious lymphocytic infiltration around the bronchi and blood vessels, forming a sleeve. Tubular phenomenon and interstitial bronchopneumonia.

　　(3) Comparative medicine In the Mycoplasma pneumoniae model rat model replicated by this method, most of the model rats were infected with the international standard 1000000 CCU/ml Mycoplasma pneumoniae strain 3 days after intranasal inoculation of the model animal. Pneumonia-related pneumonia. When the activity of Mycoplasma pneumoniae decreases, the body temperature rises and the lungs appear wet and cracked. The longer the modeling time, the more severe the symptoms of various pneumonia and the death of model animals. This indicates that Mycoplasma pneumoniae is more susceptible to rat pneumonia than the mouse model. Therefore, the model can also be used to study the etiology of human Mycoplasma pneumoniae infection, observation of curative effects and screening of new drugs.