动物造模,肺结核杆菌模型 z-bio 2021-11-04 948

　　(1) Breeding method Take adult male Wistar rat 1mg/ml standard human tuberculosis strain H37Rv bacterial suspension, and mix it with normal saline, prepare 0.01mg/0.4ml bacterial suspension from rat tail vein to suspend the bacteria. The liquid is injected into the body. In the process of observing the infection of model animals, bacteria were regularly isolated and cultured. The animals were slaughtered, tissues and organs were collected, fixed with 10% formaldehyde solution under a microscope, and normal tissue sections were prepared and stained with HE. , And observe under an optical microscope.

　　(2) Model characteristics After injection and infection, model rats can see tuberculosis lesions by HE staining, acid-fast bacilli smears are positive for tuberculosis, and the lung tissue homogenate culture has obvious tuberculosis colonies. It was observed that the rats were infected with tuberculosis for 6 weeks and no animals died.

　　(3) The screening, efficacy analysis and basic research of comparative medical tuberculosis drug models are inseparable from tuberculosis animal models. In the research of tuberculosis laboratory animals, guinea pigs are generally considered to be the most ideal model animals for establishing tuberculosis animal models. However, because guinea pigs are only infected by intraperitoneal injection, various tuberculosis experiments cannot meet the research needs. Using rats to replicate pulmonary tuberculosis infection models is similar to using mice. In addition to multiple transmission routes, it avoids the disadvantages of weak mice resistance and high mortality after modeling. Rats have high resistance and are easy to collect blood samples. .. Advantages, it can better meet the needs of large-scale scientific research. For animals of the same strain, using different transmission routes may lead to different locations and disease levels. Tail vein infection can significantly increase the weight factors of liver, spleen, lung and kidney organs in model animals, but can significantly reduce the weight of the heart. After intraperitoneal injection, the model animals only showed an increase in the weight factors of the liver and spleen organs, but the inguinal lymph nodes increased significantly.