动物造模,生殖道衣原体感染模型 z-bio 2021-04-07 336

　　(1) Copy method Hala-229, in which 30μl SPG solution is dissolved in 6 to 8 weeks old BALB/cAnN (H-2d), C3H/HeN (H-2k) and C57BL/6N (H-2b) mice Inject cell culture 3 × 10000000 IFU/ml Chlamydia MoPnNiggII strain. Five days after infection, vaginal cultures of all animals were positive for chlamydia, BALB/c and C57BL/6 mice were positive for chlamydia for 5 weeks, and C3H mice were for 4 weeks.

　　(2) Model features This model simulates the natural situation of human genital chlamydia infection. Vaginal inoculation of MoPn without progesterone can establish a model of genital chlamydia infection in mice of various strains. You can copy the mouse to the same thing. The symptoms of epidemic cervicitis, salivitis and hydrosalpinitis are easy to operate, the modeling time is short, and the success rate is high.

　　(3) The clinical symptoms and severity of symptoms of the model established by comparative medicine in this way depend on the amount of infection of various CT strains and the amount of pathogen inoculation. At the same time, the clinical symptoms of various strains of mice are: moreover, it is slightly different. Clinically, infection of human female genitals with Chlamydia trachomatis can cause urethritis, cystitis, endometritis, endometritis, pelvic infection and infertility. Although the human CT strain was used for vaginal infections in mice, vaccination with 1,000,000 IFU can only cause mild germline infections. CT mouse pneumonia type (MoPn) strain is a biological variant of CT mice. When infecting mice, MoPn is more toxic than human strains. Vaginal MoPn only requires 10,000 IFU, which usually causes cervicitis and salivitis in mice. The pregnancy rate is even infertile. Infections caused by intravaginal injection of MoPn can infect upper genital tract tissues from the lower genital tract. Inflammation and sequelae of infection after MoPn infection are the characteristics of CT infection of female genital tract. When CT human strains are used for vaginal inoculation of mice, they only cause mild lower reproductive tract infections. Only when high doses are directly inoculated into the uterine horns or ovarian sac, the human strain can cause infection sequelae in mice. This is natural to humans. The route of infection is different. Therefore, most studies currently use MoPn strains to replicate mouse and rat reproductive tract infection models, but the disadvantage of rodent models is that the IFN-γ response is different from that of human strains, and the response of this site to cytokines is limited.