动物造模,结膜炎模型 z-bio 2021-04-07 361

　　Scratching of the rabbit’s lower eyelid conjunctiva caused by inoculation with Staphylococcus aureus caused infectious inflammation of the eye conjunctiva.

　　1. How to model New Zealand white rabbits, weighing 2.2-2.5 kg, and hermaphrodite. The day before the experiment, Staphylococcus aureus was inoculated into blood agar medium, incubated at 37°C for 18 hours, and then diluted with 3×109/ml Staphylococcus aureus in normal saline for use. Take a healthy New Zealand white rabbit without eye diseases, and use No. 9 sterile to cross-mark the rabbit's lower eyelid conjunctiva. Gently pull the eyelid into a small cup, and then use a 0.25 ml syringe to inject 0.1 ml of the bacterial solution into the conjunctival scar. Twenty-four hours after infection, the eyes of each rabbit were graded according to the degree of inflammation, and randomized into groups for treatment. Give rabbits for 4 consecutive days. The rabbit eyes were scored every 24 hours and statistically processed. The scoring criteria for conjunctivitis lesions are: no abnormal blood vessels, 0 points; vascular congestion is bright red, mild edema, a small amount of secretions, 1 point; vascular congestion is purple, blood vessels should not be distinguished, obvious edema, and partial eversion of the eyelid The secretion becomes wet or makes the eyelid sticky, 2 points; hyperemia is purple, until the eyelid is almost closed and swollen, and the secretion makes the entire eyelid sticky, 3 points.

　　2. Observation indicators and analysis

　　(1) Observation of symptoms: After bacteria infect the eyelids of animals, inflammatory reactions, conjunctival hyperemia, edema and secretions increase.

　　(2) Bacterial culture test: It is performed one day after the eyelid is inoculated with bacteria, and on the first and fourth days of administration. Gently wipe the upper and lower eyelids with a cotton ball dipped in sterile saline, put in 5 ml saline and suspend evenly (test solution), take out 0.5 ml test solution, prepare ordinary agar, and then add it to the middle plate. Spread evenly and add to 37°C. Incubate for 18 hours in an incubator to determine negative and positive bacterial cultures. In the model group, the bacterial transformation rates at 1d, 2d, 5d and 8d after eyelid inoculation were about 0, 33.3%, 75.0% and 66.7%, respectively. (3) Pathological examination of conjunctiva: animals were sacrificed 4 or 5 days after bacterial inoculation, and conjunctival tissues were collected for pathological observation. In the model group, the conjunctiva was dilated, hyperemia, and edema.