动物造模,牙髓炎疼痛动物模型 z-bio 2021-04-07 870

　　1. Modeling materials Animals: 5-6 months old SD rats, male or female, weighing 200-250 g; medicine: bacterial lipopolysaccharide (LPS), 5% formaldehyde; instrument: rectal mirror.

　　2. Modeling method Use bacterial endotoxin (LPS) and sterile saline to make a 2.5 g/L LPS solution, and soak the sterile small pieces of paper in the LPS solution for 2 hours before use.

　　The module weighs the animals separately, injects the anesthetic into the abdominal cavity, disinfects, opens the pulp of the maxillary lateral incisor, rinses with saline, blots dry, inserts the LPS-impregnated paper tip into the pulp cavity, puts it in and seals it with zinc oxide. Animals in the experimental control group were directly sealed with zinc oxide. After sealing the tip of the LPS paper, the animal will survive for 24 hours. Under ether anesthesia, all animals were repeatedly stimulated on the labial side of the maxillary incisor with a hot amalgam filling head, and the animal's response after stimulation and the response after awakening were observed.

　　3. The principle of modeling is animals. They use bacterial lipopolysaccharide to induce inflammation of the rat's dental pulp, and then use thermal stimulation to induce the development of pain. This is more consistent with the clinical toothache process and is to establish a model. 4. The normal group is normal pulp tissue. In the experimental control group, the pulp tissue was almost normal, and the blood vessels were only slightly dilated. In this model, the position of the pulp cells in the pulp tissue is as follows: chaotic and empty. Pulp degeneration, reticular atrophy, vasodilation, hyperemia, inflammatory cell wall adhesion, exudation, tissue edema, etc. The rats in the normal group showed no obvious response to rapid thermal stimulation under ether anesthesia. About 5 minutes later, the mouse woke up, walked normally, and did not respond to shoes. The rats in the experimental control group did not respond significantly to heat stimulation after ether anesthesia, and after about 5 minutes, the rats woke up normally without a shoe response. Thermal stimulation of the model animals under ether anesthesia showed that the rats showed a fierce fighting response and woke up quickly. After waking up, he will hiss and collide with the cage.