冠心病,动物模型 z-bo 2022-02-06 567

　　Coronary heart disease belongs to the category of "chest obstruction and heartache" in traditional Chinese medicine, and Qi deficiency and blood stasis syndrome is one of its main symptoms, such as overwork, improper diet, overeating fat and sweet, damage to the spleen and stomach, or old age and physical weakness, visceral function weakness , Righteous Qi deficiency, Qi deficiency and blood stasis, block the heart.

　　[Modeling mechanism] At present, in the basic research of coronary heart disease, most animal models of coronary heart disease adopt single factor modeling, namely acute myocardial ischemia model or hyperlipidemia model, which are not enough to reflect the clinical characteristics of coronary heart disease. Therefore, this article introduces the modeling method of integrated traditional Chinese and Western medicine compound model.

　　[Modeling method] Experimental animals: New Zealand rabbits. 60g of high-fat feed (containing 1g of cholesterol, 15g of egg yolk powder, 5g of lard) per day, plus ordinary feed for 8 weeks, to cause food-induced atherosclerosis. According to the theory of "qi and blood are interdependent" and "heart governs blood vessels" in traditional Chinese medicine, starting from the second week of model building, the model group will have 15ml bloodletting from the ear veins twice a week, until the end of 8 weeks, in order to achieve the purpose of bleeding and gas consumption.

　　[Model features] In the 9th week after modeling, the rabbits appeared fatigue, fatigue, sluggishness, warmth, pale tongue or light fat with tooth marks, weak pulse; some of them had hairy hair, skin nails, lips and tongue bruising , Some ecchymosis appears. Pathology of the aorta showed that a large number of foamy cells accumulated in the intima and lower muscle layer of the aorta and formed fatty plaques. Cardiology shows the formation of eccentric lipid plaques in the subepicardial myocardium, with a large amount of fat tissue filling around the blood vessels; the muscular vascular intima and all muscle layers are covered with lipids, triglycerides, total cholesterol, and high-density lipoproteins "Cholesterol gel-like substance" is replaced or the blood vessel wall fat plaque is formed, and the blood vessel cavity is highly narrow or even completely blocked. Blood biochemical analysis showed that various indicators were significantly increased in varying degrees, which met the diagnostic criteria for coronary heart disease.

　　[Model Evaluation and Application] This model adopts the method of copying a compound model of integrated traditional Chinese and Western medicine. Use high-fat diet and bloodletting to establish animal models of Qi deficiency and blood stasis syndrome. From the experimental study of blood biochemical indicators, combined with the analysis of pathological results and the clinical manifestations of rabbits, it is proved that the establishment of the model of coronary heart disease and angina pectoris with Qi deficiency and blood stasis syndrome is successful. This model is mostly used in the pathology and physiology of coronary heart disease.